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Iranian Journal of Medical Sciences، جلد ۴۱، شماره ۵، صفحات ۴۰۶-۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی In Silico Analysis of Glutaminase from Different Species of Escherichia and Bacillus
چکیده انگلیسی مقاله Background: Glutaminase (EC 3.5.1.2) catalyzes the hydrolytic degradation of L-glutamine to L-glutamic acid and has been introduced for cancer therapy in recent years. The present study was an in silico analysis of glutaminase to further elucidate its structure and physicochemical properties. Methods: Forty glutaminase protein sequences from different species of Escherichia and Bacillus obtained from the UniProt Protein Database were characterized for homology search, physiochemical properties, phylogenetic tree construction, motif, superfamily search, and multiple sequence alignment. Results: The sequence level homology was obtained among different groups of glutaminase enzymes, which belonged to superfamily serine-dependent β-lactamases and penicillin-binding proteins. The phylogenetic tree constructed indicated 2 main clusters for the glutaminases. The distribution of common β-lactamase motifs was also observed; however, various non-common motifs were also observed. Conclusion: Our results showed that the existence of a conserved motif with a signature amino-acid sequence of β-lactamases could be considered for the genetic engineering of glutaminases in view of their potential application in cancer therapy. Nonetheless, further research is needed to improve the stability of glutaminases and decrease their immunogenicity in both medical and food industrial applications.
کلیدواژه‌های انگلیسی مقاله Escherichia, Bacillus, Glutaminase, Computer simulation
نویسندگان مقاله cambyz irajie | cambyz irajie
department of public health and food hygiene, school of veterinary medicine, shiraz university, shiraz, iran

سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

میلاد محکم | milad mohkam
pharmaceutical sciences research center, shiraz university of medical sciences, shiraz, iran; and department of pharmaceutical biotechnology, school of pharmacy, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

نوید نظافت | navid nezafat
pharmaceutical sciences research center, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

سعید حسین زاده | saeed hosseinzadeh
department of public health and food hygiene, school of veterinary medicine, shiraz university, shiraz, iran

سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

محمود امین لاری | mahmood aminlari
department of biochemistry, school of veterinary medicine, shiraz university, shiraz, iran

سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

یونس قاسمی | younes ghasemi
pharmaceutical sciences research center, shiraz university of medical sciences, shiraz, iran; and department of pharmaceutical biotechnology, school of pharmacy, shiraz university of medical sciences, shiraz, iran; and department of medical biotechnology, school of advanced medical sciences and technologies, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

نشانی اینترنتی http://ijms.sums.ac.ir/index.php/IJMS/article/view/2225
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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