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Iranian Journal of Medical Sciences، جلد ۴۱، شماره ۵، صفحات ۴۲۲-۰
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عنوان انگلیسی Prevalence of the Most Common Virulence-Associated Genes among Brucella Melitensis Isolates from Human Blood Cultures in Hamadan Province, West of Iran
چکیده انگلیسی مقاله Brucellosis is a widespread zoonotic disease causing considerable economic and public health problems. Despite animal vaccination, brucellosis remains endemic in some areas such as Iran, especially in the western Iranian province of Hamadan. We sought to detect some of the most common virulence-associated genes in Brucella isolated from human blood cultures to determine the prevalence of some virulence genes among Brucella isolates. Fifty-seven isolates were studied from patients with a clinical diagnosis of brucellosis who referred to the Infectious Diseases Ward of Sina Hospital in Hamadan Province, Iran, between April 2013 and July 2014. Blood samples were collected for the diagnosis of brucellosis using the BACTEC blood culture system. All of these isolates were confirmed by the bcsp31 Brucella-specific gene. We detected 11 virulence-associated genes of Brucella, namely cβg, virB, znuA, ure, bvfA, omp25, omp31, wbkA, mviN, manA, and manB, which are important for the pathogenesis of this bacterium in the intracellular environment by multiplex PCR. Totally, 149 patients with a clinical diagnosis of brucellosis were enrolled in this study. Fifty-seven (38.3%) patients had positive blood cultures. On biochemical and molecular testing, all of the isolates were Brucella melitensis. Ten of the virulence genes were detected among all of the 57 isolates, but the bvf gene was detected in 53 (93%) isolates. The high prevalence of virulence-associated genes among the Brucella isolates detected in Hamadan Province, Iran, underscores the pathogenicity of this bacterium in this region.
کلیدواژه‌های انگلیسی مقاله Virulence factors, Brucella melitensis, Brucellin, Iran
نویسندگان مقاله ناصری | zahra naseri
brucellosis research center, hamadan university of medical sciences, hamadan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)

محمد یوسف علیخانی | mohammad yousef alikhani
department of microbiology, faculty of medicine, hamadan university of medical sciences, hamadan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)

سید حمید هاشمی | seyed hamid hashemi
brucellosis research center, hamadan university of medical sciences, hamadan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)

فریده کمره یی | farideh kamarehei
department of microbiology, faculty of medicine, hamadan university of medical sciences, hamadan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)

محمد رضا عربستانی | mohammad reza arabestani
department of microbiology, faculty of medicine, hamadan university of medical sciences, hamadan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)

نشانی اینترنتی http://ijms.sums.ac.ir/index.php/IJMS/article/view/1655
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Brief Report(s)
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