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Iranian Journal of Medical Sciences، جلد ۴۱، شماره ۴، صفحات ۲۷۵-۰
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عنوان انگلیسی Effects of Ketamine on Neuronal Spontaneous Excitatory Postsynaptic Currents and Miniature Excitatory Postsynaptic Currents in the Somatosensory Cortex of Rats
چکیده انگلیسی مقاله Background: Ketamine is a commonly used intravenous anesthetic which produces dissociation anesthesia, analgesia, and amnesia. The mechanism of ketamine-induced synaptic inhibition in high-level cortical areas is still unknown. We aimed to elucidate the effects of different concentrations of ketamine on the glutamatergic synaptic transmission of the neurons in the primary somatosensory cortex by using the whole-cell patch-clamp method. Methods: Sprague-Dawley rats (11–19 postnatal days, n=36) were used to obtain brain slices (300 μM). Spontaneous excitatory postsynaptic currents (data from 40 neurons) were recorded at a command potential of -70 mV in the presence of bicuculline (a competitive antagonist of GABAA receptors, 30 μM) and strychnine (glycine receptor antagonist, 30 μM). Miniature excitatory postsynaptic currents (data from 40 neurons) were also recorded when 1 μM of tetrodotoxin was added into the artificial cerebrospinal fluid. We used GraphPad Prism5for statistical analysis. Significant differences in the mean amplitude and frequency were tested using the Student paired 2-tailed t test. Values of P< 0.05 were considered significant. Results: Different concentrations of ketamine inhibited the frequency and amplitude of the spontaneous excitatory postsynaptic currents as well as the amplitude of the miniature excitatory postsynaptic currents in a concentration-dependent manner, but they exerted no significant effect on the frequency of the miniature excitatory postsynaptic currents. Conclusion: Ketamine inhibited the excitatory synaptic transmission of the neurons in the primary somatosensory cortex. The inhibition may have been mediated by a reduction in the sensitivity of the postsynaptic glutamatergic receptors.
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نویسندگان مقاله chengdong yuan | chengdong yuan
guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china


yajun زانگ | yajun zhang
guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china


yu زانگ | yu zhang
guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china


song cao | song cao
guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china


yuan ونگ | yuan wang
guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china


bao fu | bao fu
guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china


tian yu | tian yu
guizhou key laboratory of anesthesia and organ protection, zunyi medical college, guizhou, china
نشانی اینترنتی http://ijms.sums.ac.ir/index.php/IJMS/article/view/2533
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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