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Iranian Journal of Medical Sciences، جلد ۳۹، شماره ۴، صفحات ۳۵۰-۰
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عنوان انگلیسی IL-23/IL-27 Ratio in Peripheral Blood of Patients with Breast Cancer
چکیده انگلیسی مقاله Background: Interleukin (IL)-23 and IL-27 are two IL-12-related cytokines which their function may dramatically influence the inflammatory response to tumor development. IL-12 and IL-27 seem to have antagonistic roles with IL-23 in tumor site. In this study, IL-23 and IL-27 mRNA expressions were analyzed in peripheral blood of patients with breast cancer and healthy volunteers using quantitative real-time PCR. Methods: Peripheral blood samples were collected from 50 women with breast cancer and 50 healthy ones. The total RNA was extracted from peripheral blood after lysis with ammonium chloride and TRizol reagent and the cDNA was synthesized. The expression of IL-23 and IL-27 gene transcripts was determined with real-time polymerase chain reaction (qRT-PCR) using Syber Green PCR Master Mix. Results: It is found that IL-23 and IL-27 transcripts had significantly higher expression in peripheral blood of patients compared with the healthy controls. The ratio of IL-23 transcript expression to IL-27 was 3.4 fold lower in the studied patients compared with the normal individuals. Conclusion: It is concluded that the over expression of IL-23 and IL-27 gene transcript in peripheral blood of breast cancer patients may be an immune response against tumor development and the inflammatory response plays a critical role in tumor development via up regulating the corresponding cytokines. However, the IL-23/IL-27 ratio may play an important role in cytokine-based immunotherapy against cancer. Further research should be carried out to assess these cytokines in a larger sample size.
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نویسندگان مقاله علی خدادادی | ali khodadadi
department of immunology, ahvaz jondishapur university of medical sciences, ahvaz, iran


محبوبه رزمخواه | mahboobeh razmkhah
institute for cancer research, school of medicine, shiraz university of medical sciences, shiraz, iran;

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

علی رضا اسکندری | ali reza eskandari
department of immunology, ahvaz jondishapur university of medical sciences, ahvaz, iran


دکتر احمد حسینی | ahmad hosseini
institute for cancer research, school of medicine, shiraz university of medical sciences, shiraz, iran;

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

مجتبی حبیب آگهی | mojtaba habibagahi
department of immunology, school of medicine, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

عباس قادری | abbas ghaderi
department of immunology, school of medicine, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

منصوره جابری پور | mansooreh jaberipour
institute for cancer research, school of medicine, shiraz university of medical sciences, shiraz, iran;

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

نشانی اینترنتی http://ijms.sums.ac.ir/index.php/IJMS/article/view/949
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