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Basic and Clinical Cancer Research، جلد ۵، شماره ۳، صفحات ۱۱-۱۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Expression of miR-520d in breast cancer
چکیده انگلیسی مقاله Background: Breast cancer is the most common cancer in women. Non-coding RNAsespecially miRNAs have important regulatory roles in cancer. MiRNAs are 21-24 nucleotides which have different levels of expression between tumors and normal tissues. In this study, we have analyzed expression level of miR-520d in three different groups of breast cancer. Methods: Fifty nine samples were divided into different groups according to their immunohistochemistry (IHC) classification: estrogen receptor (ER) positive and/or progesterone receptor (PR) positive group (as group I); human epidermal growth factor receptor 2 (HER2) positive group (as group II); and Triple negative group (as group III). After small RNA extraction from tissues, cDNAs were synthesized and Real time RTPCR carried out using DNA binding dye. Expression levels were analyzed by LinRegPCR and REST software. Results: MiR-520d under- expressed in all of three different groups. The expression ratio in groups I ,II, and III were 0.193, 0.167, 0.21, respectively but only the result from group II was significant (P=0.017). According to the different clinic-pathological status of breast cancer, miR-520d under-expressed significantly not only in patients with metastatic lymph node (P=0.019) but also in patients which have cancer at stage III (P=0.036). conclusion: In this study, we found that miR-520d possibly acts as a tumor suppressor. It may be useful for diagnosis of tumor from normal tissue. In addition, miR-520d significantly under-expressed in HER-2 positive group of breast cancers. Therefore, it may be useful as an additional diagnostic test in this group of breast tumors along with other biomarkers.
کلیدواژه‌های انگلیسی مقاله Breast cancer,miR-520d,real time RT-PCR,SYBR green I Dye
نویسندگان مقاله شهرام سواد | shahram savad
medical geneticist, alborz university of medical sciences, alborz, iran


پروین مهدی پور | parvin mahdipour
department of medical genetics, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

هدی شیردست | hoda shirdast
department of medical microbiology, qazvin university of medical sciences, qazvin, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (Qazvin university of medical sciences)

لادن نکوهش | ladan nekoohesh
general practitioner


لیلی نکوهش | leili nekoohesh
general practitioner


رضا شیرکوهی | reza shirkoohi
department of genetics and genomics, cancer research center, tehran, iran


وحید نیکویی | vahid nikooee
department of pharmacology, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمد میریونسی | mohammad miryounesi
genomic research center, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

محمد حسن مدرسی | mohammad hasan modarresi
department of medical genetics, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://bccr.tums.ac.ir/index.php/bccrj/article/view/96
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Articles
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