| چکیده انگلیسی مقاله |
Background and Aim: Understanding the molecular mechanisms involved in the increased levels of HbF inducing drugs should be advised for effective induction. The aim of this study was to investigate the molecular effects of the drugs thalidomide and sodium butyrate considered as HbF inducer agents. Materials and Methods: In this experimental study, CD133+ cord blood stem cells carrying mutations of heterozygous β-thalassemia were isolated and differentiated into erythroid lineage. In order to evaluate the expression of the erythroid markers, CD71 and CD235a, was analysed. For this purpose, the RNA extracted from erythroid precursors at days 6 and 12 of erythroid differentiation and cDNA synthesized, and then the expression of these genes was performed by quantitative Real-time PCR technique. Results: The results of this study showed the significant effect of thalidomide on erythroid proliferation as compared to sodium butyrate and control group (P< 0.05). Also, thalidomide significantly increased CD71expression and decreased CD235a expression as compared to sodium butyrate and control groups (P< 0.05). Conclusion : Thalidomide may play its role on HbF induction by increasing the proliferation of early erythroid precursors. |
| نویسندگان مقاله |
زهرا کاشانی خطیب | zahra kashani khatib
master of sciences student in hematology, hematology department, school of medical sciences, tarbiat modares university amp;amp; students scientific research center ssrc , tehran university of medical sciences, tehran, iran
دانشکده علوم پزشکی، دانشگاه تربیت مدرس و عضو مرکز پژوهشهای دانشجویی، دانشگاه علوم پزشکی تهران
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
علی دهقانی فرد | ali dehghanifard
ph.d student in hematology, hematology department, school of medical sciences, tarbiat modares university and sarem cell research center scrc , sarem women’s hospital, tehran, iran
مرکز تحقیقات سلولی- مولکولی و سلول های بنیادی صارم، بیمارستان فوق تخصصی صارم
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
سعید کاویانی | saeid kaviani
associate professor, hematology department, school of medical sciences, tarbiat modares university, tehran, iran
دانشکده علوم پزشکی، دانشگاه تربیت مدرس
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
مهرداد نوروزی نیا | mehrdad noruzinia
associate professor, medical genetics department, school of medical sciences, tarbiat modares university, tehran, iran
دانشکده علوم پزشکی، دانشگاه تربیت مدرس
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
مومنه محمدی | momeneh mohammadi
ph.d student in hematology, hematology department, school of medical sciences, tarbiat modares university, tehran, iran
دانشکده علوم پزشکی، دانشگاه تربیت مدرس
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
فاطمه محمد علی | fatemeh mohammad ali
ph.d student in hematology, hematology department, school of medical sciences, tarbiat modares university, tehran, iran
دانشکده علوم پزشکی، دانشگاه تربیت مدرس
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
الهام روشندل | elham roshandel
ph.d student in hematology, hematology department, school of medical sciences, tarbiat modares university, tehran, iran
دانشکده علوم پزشکی، دانشگاه تربیت مدرس
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
سحر محمدی فاتح | sahar mohammadi fateh
bachelor of sciences in medical laboratory, sarem cell research center scrc , sarem women’s hospital, tehran, iran
مرکز تحقیقات سلولی-مولکولی و سلول های بنیادی صارم، بیمارستان فوق تخصصی صارم
شعبان علیزاده | shaban alizadeh
assistant professor, hematology and transfusion medicine department, school of allied medicine, tehran university of medical sciences, tehran, iran
دانشکده پیراپزشکی، دانشگاه علوم پزشکی تهران
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
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