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Acta Medica Iranica، جلد ۵۶، شماره ۷، صفحات ۴۲۱-۴۲۸
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عنوان انگلیسی Acute Transplantation of Human Olfactory Mucosa-Derived Olfactory Ensheathing Cells Fails to Improve Locomotor Recovery in Rats
چکیده انگلیسی مقاله Olfactory ensheathing cells-based therapy for spinal cord injury (SCI) repair has been a possible treatment for clinical study because of their safety in autologous transplantation and potential regenerative capability. However, there are contradictory reports on the results after transplantation in animal models. The purpose of this research was to investigate the effect of acute transplantation of human mucosa-derived olfactory ensheathing cells (OECs) on the repair of the spinal cord. Human olfactory ensheathing cells were isolated from the human mucosa and cultured under supplemented neuronal cell culture medium. They were characterized by immunocytochemistry for olfactory ensheathing cell markers. We induced spinal cord injury at T8-T9 of rats by aneurysm clips and simultaneously injected two million OECs into subarachnoid space of spinal cord. Sensory and motor behaviors were recorded by tail-flick reflex (TFR) and BBB scores, respectively every week for seven weeks after injury. Morphology and S100-beta antigen expression in olfactory ensheathing cells of the human olfactory mucosa was confirmed by immunostaining . OECs transplantation did not recover inflammation, neuronal vacuolation, hemorrhage, and cyst formation. These findings suggest that OECs transplantation in this experimental setting did not lead to tissue regeneration to enhance locomotion. These results broaden current knowledge and are additions to the science and literature.
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نویسندگان مقاله | Mina Afhami
Department of Neuroscience, Functional Neurosurgery Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.


| Mansoureh Hashemi
Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran.


| Khashayar Afshari
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.


| Jamal Chenari
Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.


| Seyed Mohammad Tavangar
Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran. ANDDepartment of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.


| Mahmoudreza Hadjighassem
Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Pharmacology, Tehran University of Medical Sciences, Tehran, Iran.


| Ahmad reza Dehpour
Cancer Biology Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.


| Ahad Muhammadnejad
Department of Pharmacology, Tehran University of Medical Sciences, Tehran, Iran. AND Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran.


| Arvin Haj-Mirzaian
Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran. AND 10 Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran. AND. Department of Neurosurgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.


| Vafa Rahimi-Movaghar


نشانی اینترنتی http://acta.tums.ac.ir/index.php/acta/article/view/6754
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/56/article-56-790663.pdf
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