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Medical Laboratory Journal، جلد ۱۰، شماره ۲، صفحات ۱۹-۲۴
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Comparison of Disc Diffusion, Broth Microdilution and Modified Hodge Test Susceptibility Testing Of Escherichia coli Isolates to Beta-Lactam Antibiotics
چکیده انگلیسی مقاله ABSTRACT Background and Objective: One of the main tasks of clinical microbiology laboratories is to determine antibiotic resistance profiles in common pathogens and ensure the selection of effective antibiotics for certain infections. The aim of this study was to compare the methods of disk diffusion, broth microdilution and modified Hodge test in Escherichia coli isolates from urinary tract infection and diarrhea for susceptibility testing against beta-lactam antibiotics in Kerman, Iran. Methods: In this study, 432 E. coli isolates were collected from diarrhea (216 isolates) and urinary tract infection samples (216 isolates). The antibiotic susceptibility testing methods of disk diffusion, broth microdilution and modified Hodge test were performed according to the Clinical and Laboratory Standards Institute guidelines. Results: The findings of disk diffusion method showed that resistance to cefotaxime, ceftazidime, aztreonam, cefepime and imipenem was 51.15%, 30.55%, 24.30%, 15.27% and 1.85%, respectively. In the disk diffusion test, 51.15% of the isolates were resistant to at least one antibiotic, all of which were later evaluated by the broth microdilution method. Moreover, 52.94%, 17.19%, 13.12% and 0.90% of the isolates were resistant to cefotaxime, ceftazidime, cefepime and imipenem, respectively. All of the isolates were evaluated for the production of carbapenemase enzyme by the modified Hodge test and none of the isolates were found as positive. Conclusion: This study shows that performing carbapenem tests is very challenging, and laboratories are recommended to use secondary and independent antibiotic susceptibility tests such as modified Hodge test to confirm the carbapenem-resistant results.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله حسام علیزاده | hesam alizade
department of microbiology


فاطمه فلاح | fatemeh fallah
department of microbiology


رضا قنبرپور | reza ghanbarpour
department of microbiology


حسین گودرزی | hosein goudarzi
department of microbiology


حمید شریفی | hamid sharifi
modeling in health research center


محمدرضا افلاطونیان | mohammad reza aflatoonian
research center for tropical and infectious diseases
نشانی اینترنتی http://www.goums.ac.ir/mljgoums/browse.php?a_code=A-10-1-390&slc_lang=fa&sid=fa
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زبان مقاله منتشر شده en
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