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Iranian Journal of Basic Medical Sciences، جلد ۲۱، شماره ۱۰، صفحات ۹۹۲-۹۹۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Kalirin-7 plays the neuroprotective role in Neuro-2A cells injured by oxygen-glucose deprivation and reperfusion through Rac1 activation
چکیده انگلیسی مقاله Objective(s): The study explored the neuroprotective role of Kalirin-7 (Kal-7) in Neuro-2A cells after oxygen-glucose deprivation and reperfusion (OGD/R) treatment.Materials and Methods: The study used an OGD/R model of mouse Neuro-2A neuroblastoma cells in vitro. Cells were transfected with pCAGGS-Kal-7 to up-regulating kal-7. Then cell proliferation and apoptosis were respectively analyzed by Trypan blue exclusion method and flow cytometry. To examine the involvement of Rac1, cells were treated with Rac1-GTP inhibitor NSC23766 before treatment with OGD/R. Expressions of Bax, Bcl-2, Rac1, and down-stream targets of Rac1 were analyzed by Western blot.Results: Kal-7 significantly decreased OGD/R induced cell apoptosis (P< 0.01), but no significant effects were observed on cell proliferation. Kal-7 increased the expressions of apoptosis-related protein of Bcl-2 and Rac1, but decreased the expression of Bax in Neuro-2A cells stimulated to OGD/R. Rac1 was activated by Kal-7 due to the increased levels of its down-stream targets, p-p38 and p-PAK1. NSC23766 reduced the anti-apoptotic effect of Kal-7 as the enhanced apoptotic cell rate and increased Bax/Bcl-2 ratio. Conclusion: These findings suggest that the protective effects of Kal-7 against OGD/R injury in Neuro-2A cells were dependent in a Rac1 activation signaling.
کلیدواژه‌های انگلیسی مقاله Anti-apoptosis, Kalirin-7, Neuroprotection, OGD/R, Rac1
نویسندگان مقاله | Jian Xu
Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao 266500, China


| Yanxiu Chen
Department of Neurology, Liaocheng People’s Hospital, Liaocheng 252000, China


| Zeyu Wu
Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao 266500, China


| Yihe Dou
Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao 266500, China


| Peng Lun
Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao 266500, China


| Peng Sun
Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao 266500, China
نشانی اینترنتی http://ijbms.mums.ac.ir/article_11362.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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