| چکیده انگلیسی مقاله |
Introduction: recurrent implantation failure (RIF) is the most common cause of pregnancy failure after in vitro fertilization (IVF). Among the different causes of RIF, the role of maternal genetics factors is as one of most important factors. P53 codon 72 polymorphism is one of the notable polymorphisms in this field. According to evidences about the association between P53 polymorphism and increased risk of RIF in different population, if this relationship be justified in Iranian population, this polymorphism can be used to predict IVF success rate. This study was performed with aim to evaluate the association between this polymorphism and incidence of RIF. Methods: This case-control study was performed on 80 infertile couples in Mashhad Montaserieh Infertility Treatment Center in 2010. The infertile couples were divided into three groups of control, RIF-1, and RIF-2. The control group included 40 women who were successfully pregnant after IVF. The patient group 1 were 20 women who no type of pregnancy was observed after two IVF, and patient group 2 were 20 women who failed to be pregnant after at least three IVF. P53 gene polymorphism genotype (Arg72pro allele) was determined using PCR- ARMS technique. Statistical analysis was performed by SPSS software (version 16) and Pearson Chi-Square test. P< 0.05 was considered significant. Results: The frequency of Arg/Pro genotype in control group, patient group 1, patient group 2 and total patients was 92.5%, 75%, 80% and 77.5%. The frequency of Arg/Arg genotype in these groups was 7.5%, 25%, 20% and 22.5%, respectively. Pro/Pro genotype was observed in none of the groups. According to Pearson Chi-square test results, distribution of genotypes was not significantly different between patient group 1 and patient group 2 and also total patients in comparison with control group. Conclusion: According to the results of present study in the studied population, there is no association between P53 codon 72 polymorphism and occurrence of RIF. So this polymorphism cannot be used to predict the risk of RIF. |
| نویسندگان مقاله |
سمیه علافان | somayeh allafan
msc student, department of biology, school of basic sciences, mashhad branch, islamic azad university, mashhad, iran.
دانشجوی کارشناسی ارشد زیست شناسی سلولی تکوینی، دانشکده علوم پایه، دانشگاه آزاد اسلامی مشهد، مشهد، ایران
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی مشهد (Islamic azad university of mashhad)
محمد حسن زاده نظرآبادی | mohammad hasanzadeh nazarabadi
professor, department of medical genetics, school of medicine, mashhad university of medical sciences, mashhad, iran.
استاد گروه ژنتیک پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
محبوبه انقلابی فر | mahbubeh enghelabifar
msc student, department of biology, school of basic sciences, mashhad branch, islamic azad university, mashhad, iran.
دانشجوی کارشناسی ارشد زیست شناسی سلولی تکوینی، دانشکده علوم پایه، دانشگاه آزاد اسلامی مشهد، مشهد، ایران.
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی مشهد (Islamic azad university of mashhad)
جینا خیاط زاده | jina khayatzadeh
assistant professor, department of biology, school of basic sciences, mashhad branch, islamic azad university, mashhad, iran.
استادیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی مشهد، مشهد، ایران.
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی مشهد (Islamic azad university of mashhad)
خدیجه شاهرخ آبادی | khadijeh shahrokh abadi
assistant professor, department of biology, school of basic sciences, mashhad branch, islamic azad university, mashhad, iran.
استادیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی مشهد، مشهد، ایران
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی مشهد (Islamic azad university of mashhad)
محسن جلالی | mohsen jalali
physician, montaserieh infertility treatment center, mashhad university of medical sciences, mashhad, iran.
پزشک، مرکز تحقیقاتی و درمان ناباروری منتصریه، دانشگاه علوم پزشکی مشهد، مشهد، ایران.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
فهیمه مرادی | fahimeh moradi
msc student, department of human genetics, school of medicine, mashhad university of medical sciences, mashhad, iran.
دانشجوی کارشناسی ارشد ژنتیک انسانی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
نزهت موسوی فر | nozhat musavifar
assistant professor, department of obstetrics and gynecology, women apos;s health research center, school of medicine, mashhad university of medical sciences, mashhad, iran.
استادیار گروه زنان و مامایی، مرکز تحقیقات سلامت زنان، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
جواد زوار رضا | javad zavar reza
assistant professor, department of biochemistry, school of medicine, yazd shahid sadoughi university of medical sciences, yazd, iran.
استادیار گروه بیوشیمی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید صدوقی یزد، یزد، ایران
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید صدوقی یزد (Shahid sadooghi university of medical sciences)
مجید مجرد | majid mojarrad
assistant professor, department of medical genetics, school of medicine, mashhad university of medical sciences, mashhad, iran.
استادیار گروه ژنتیک پزشکی، مرکز تحقیقات ژنتیک پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
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