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Cell Journal، جلد ۲۰، شماره ۳، صفحات ۳۱۲-۳۱۷
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عنوان انگلیسی Identification of An mtDNA Setpoint Associated with Highest Levels of CD44 Positivity and Chemoresistance in HGC-27 and MKN-45 Gastric Cancer Cell Lines
چکیده انگلیسی مقاله Objective: Cancer stem cells have important roles in survival and chemoresistance, and CD44 and CD24 are common markers of cancer stem cells. In our study, we aimed to analyze the effects of mtDNA levels on the cell surface positivity to anti-CD24 and anti-CD44 antibodies and the chemoresistance (5-FU and cisplatin) in AGS, HGC-27 and MKN-45 gastric cancer cells and to determine a setpoint for mtDNA levels for same cells. Materials and methods: In this study, we decreased mtDNA levels in AGS, HGC-27 and MKN-45 gastric cancer cell lines by EtBr treatment. mtDNA depletion was confirmed with quantitative RT-PCR. Changes in cell surface positivity to anti-CD24 and anti-CD44 antibodies in control and mtDNA depleted AGS, HGC-27 and MKN-45 cells were analyzed with flow cytometry. In addition, changes in chemoresistance (5-FU and cisplatin) were analyzed for same cells. The relation between mtDNA level, cell surface positivity to anti-CD24 and anti-D44 antibodies were determined. Results: The highest CD44 positivity was found in HGC-27 and MKN-45 ρlow cells which have 33-40% of mtDNA of those in control cells while mtDNA depletion decreased CD24 positivity in same cells. The highest chemoresistance was found in all ρlow cells which have 33-40% of mtDNA of those in control cells. mtDNA recovered (reverted) HGC-27 and MKN-45 cells partially maintained their increased chemoresistance while reverted AGS cells could not maintain increased chemoresistance. Conclusion: mtDNA depletion trigger chemoresistance in correlation with the increase in CD44 positivity and the decrease in CD24 positivity in HGC-27, MKN-45 metastatic gastric cancer cells. The level of mtDNA depletion was significantly affected the level of CD44 positivity and chemoresistance. When the mtDNA level rose above or fell below a certain value (33-40% of that in control cells), the levels of CD44 positivity and chemoresistance were decreased.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Gökhan Terzioğlu


| Özlem Türksoy


| Ömer Faruk Bayrak


نشانی اینترنتی http://celljournal.org/journal/article/abstract/5309
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/16/article-16-851775.pdf
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