این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۲۰، شماره ۳، صفحات ۳۲۶-۳۳۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Chronic Obstructive Pulmonary Disease Molecular Subtyping and Pathway Deviation-Based Candidate Gene Identification
چکیده انگلیسی مقاله Objective: This study was intended to identify the molecular subtypes of chronic obstructive pulmonary disease (COPD) and to explore the candidate genes for this disease using bioinformatics methods. Materials and methods: The gene expression profiling GSE76705 (including 229 COPD samples) and known COPD-related genes (candidate genes) were downloaded from Gene Expression Omnibus (GEO) and Online Mendelian Inheritance in Man (OMIM) databases, respectively. According to the expression values of the candidate genes in COPD samples, these COPD samples were divided into several molecular subtypes through hierarchical clustering analysis. The candidate genes were allocated into the subtypes, and performed functional enrichment analysis. Then pathway deviation scores were analyzed, followed by clinical indicators (FEV1, FEV1/FVC, age, and gender) analysis of COPD patients in each subtype and prediction models construction. Furthermore, the gene expression profiling GSE71220 and Comparative Toxicogenomics Database (CTD) database were used to verify our results. Results: A total of 213 COPD-related genes were identified, which were divided into three subtypes. After overlaps analysis, 160 common specific genes, such as transforming growth factor β1 (TGFB1), epidermal growth factor receptor (EGFR), and interleukin 13 (IL13), were obtained. Functional enrichment analysis identified 22 specific pathways, such as hsa04060: Cytokine-cytokine receptor interaction pathways, hsa04110: Cell cycle, and hsa05222: Small cell lung cancer. Pathways in subtype 2 had higher deviation scores. Furthermore, three receiver operating characteristic curves (accuracies > 80%) were constructed. The validation data GSE71220 also identified three subtypes in COPD samples. TGFB1, EGFR, and IL13 were included in COPD associated genes in CTD database. Conclusion: COPD may be further subdivided into several subtypes. Cytokines-associated genes, such as TGFB1, EGFR, and IL13 were likely to be important candidate genes of COPD.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Jingming Zhao


| Wei Cheng


| Xigang He


| Yanli Liu


| Jiaxing Sun


| Jiaxing Sun


| Jinfeng Li


| Fangfang Wang


| Yufang Gao


نشانی اینترنتی http://celljournal.org/journal/article/abstract/5412
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/16/article-16-851777.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات