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Cell Journal، جلد ۲۰، شماره ۳، صفحات ۳۶۹-۳۷۶
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عنوان انگلیسی Morphological, Ultrastructural, and Molecular Aspects of In Vitro Mouse Embryo Implantation on Human Endometrial Mesenchymal Stromal Cells in The Presence of Steroid Hormones as An Implantation Model
چکیده انگلیسی مقاله Objective: This experimental study aimed to evaluate the effects of 17β-estradiol (E2) and progesterone (P4) on the interaction between mouse embryo and human endometrial mesenchymal stromal cells and genes expression related to implantation (αV, β3 integrins, IL-1R & LIFR) using an in vitro two dimensional model. Materials and methods: The endometrial stromal cells were isolated enzymatically and mechanically and cultured to the fourth passage then their immunophenotype was confirmed by flow cytometric analysis as mesenchymal stromal cells. Then they were cultured into experimental group in the presence of E2 (0.3 nmol) and P4 (63.5 nmol) and control group without any hormonal treatment. The mouse blastocysts were co-cultured with endometrial mesenchymal stromal cells in both groups for 48 hours. Their interaction was assessed under the inverted microscope and scanning electron microscope. The expression of αV and β3 integrins, leukemia inhibitory factor receptor (LIFR), interleukin-1 receptor (IL-1R) genes was analyzed by real time RT-PCR. Results: The similar observations were seen in both groups by light microscopy and SEM. In two groups, the presence of pinopodes-like structures and cell secretions were seen on the apical surfaces of endometrial mesenchymal stromal cells. The trophoblastic cells were expanded and interacted with the mesenchymal monolayer cells. At the molecular level the expression of IL-1R was significantly increased in hormonal treated group compared to the control (P≤0.05) and the other genes expression was not differed. Conclusion: This study showed that the co-culture of endometrial mesenchymal stromal cells with mouse embryo in the media containing E2 (0.3 nmol) and P4 (63.5 nmol) can be effective to increase the expression of IL-1R gene which is involved in the embryo implantation however have not significant effects on the expression of αV and β3 integrins and LIFR and on the morphology and ultrastructure of endometrial mesenchymal stromal cells.
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نویسندگان مقاله | Marzieh Rahimipour


| Mojdeh Salehnia


| Mina Jafarabadi


نشانی اینترنتی http://celljournal.org/journal/article/abstract/5221
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