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Cell Journal، جلد ۲۰، شماره ۳، صفحات ۴۰۳-۴۱۱
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عنوان انگلیسی The Effects of Lysophosphatidic Acid on The Incidence of Cell Death in Cultured Vitrified and Non-Vitrified Mouse Ovarian Tissue: Separation of Necrosis and Apoptosis Border
چکیده انگلیسی مقاله Objective: Objective: The cell death which induced by physical and chemical conditions during cryopreservation and / or in vitro culture had impact on follicular development. In the present study we examined whether LPA can be decreased the incidence of cell death to improve the in vitro culture conditions in cultured vitrified mouse ovarian tissue. Materials and methods: Materials & Methods:In this experimental study, 7-day-old mouse ovarian tissues were collected and divided randomly in vitrified and non-vitrified groups then the ovaries were cultured in the presence and absence of LPA for 1 week. The morphology and follicular development was evaluated by hematoxylin and eosin and masson’s trichrome staining. The incidence of cell death was assessed by flow cytometry using anexin V/Pi and caspase-3/7 assay in all studied groups. Results: Results: The developmental rate of follicles was significantly decreased in vitrified groups in comparison with non-vitrified samples (P< 0.05). The overall qualitative and quantitative results were shown that the follicular degeneration was prominent in vitrified groups compared with the respective non-vitrified groups. In addition, in both LPA treated groups, the proportion of preantral follicles was significantly higher than non-LPA treated groups (P< 0.05). The flow cytometry analysis demonstrated that totally the proportions of apoptotic (early and late) cells in all groups of study were significantly higher than necrotic cells (17.83% ± 8.80 vs 7.97% ± 0.92) (P< 0.05). In addition the percentage of these cells was significantly increased in the vitrified groups in comparison with non-vitrified samples. The LPA treated groups showed the lower percentage of these cells than non-LPA treated groups (P< 0.05). The lower enzyme activity was observed in non-vitrified (especially in LPA+ groups) cultured ovaries than vitrified group (P< 0.05). Conclusion: Conclusion: It seems that both vitrification and in vitro culture had adversely effects on cell survival and caused cell death. We postulate that LPA supplementation of culture medium could improves the developmental rate of follicles and acts as an anti-cell death factor in non-vitrified and vitrified ovarian tissues and it could be applicable for in vitro maturation of ovarian tissue.
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نویسندگان مقاله | Neda Abedpour


| Mojdeh Salehnia


| Nassim Ghorbanmehr


نشانی اینترنتی http://celljournal.org/journal/article/abstract/5180
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