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Cell Journal، جلد ۲۰، شماره ۳، صفحات ۴۱۲-۴۲۱
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عنوان انگلیسی Evaluation of Hydro-Alcoholic Extract of Trifolium Pratens L. for Its Anti-Cancer Potential on U87MG Cell Line
چکیده انگلیسی مقاله Objective: Glioblastoma multiforme is the most malignant form of brain tumors. Trifolium pratense L. has been suggested for cancer treatment in traditional medicine. Here the effect of T. pratense extract on glioblastoma multiforme cell line (U87MG) was investigated. Materials and methods: In this expremental study the effect of T. pratense extract on cell viability was investigated using trypan blue staining, MTT assay, and lactate dehydrogenase activity measurement. Apoptosis and autophagy cell death was detected by fluorescent dyes staining. Nitric oxide production was measured using Griess reaction. Expression level of some apoptotic and autophagic- related genes was detected using real-time PCR. The combination effect of T. pratense exteract and temozolomide was evaluated by calculating the combination index and dose reduction index values. Results: After treatment with T. pratense extract, the cell viability was significantly reduced in a time- and dose-dependent manner (p< 0.05). Apoptosis and autophagy of U87MG cells were significantly increased (p< 0.05). Also T. pratense extract significantly decreased NO production (p< 0.05) by U87MG cell. Combination of temozolomide and T. pratense extract had a synergistic cytotoxic effect. Conclusion: T. pratense showed an anti-cancer properties via induction of apoptosis and autophagy cell death.
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نویسندگان مقاله | Mozafar Khazaei


| Mona Pazhouhi


| Saber Khazaei


نشانی اینترنتی http://celljournal.org/journal/article/abstract/5380
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/16/article-16-851788.pdf
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