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Cell Journal، جلد ۲۰، شماره ۱، صفحات ۱۹-۲۴
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عنوان انگلیسی The Angiognic Chemokines Expression Profile of Myeloid Cell Lines Co-Cultured with Bone Marrow-Derived Mesenchymal Stem Cells
چکیده انگلیسی مقاله Objective: Angiogenesis, the process of new blood vessel formation, is essential for solid tumors development. Since acute myeloid leukemia (AML) known as a liquid tumor, at first it was thought that angiogenesis is not implicated in its development. One of the most important elements in bone marrow microenvironment is mesenchymal stem cell (MSC). These cells possess an intrinsic tropism for sites of tumor in various types of cancers and have an impact on solid tumor growth by the effect on angiogenic process. But so far, our knowledge is limited about its role in liquid tumor angiogenesis. Materials and methods: In this experimental study, the HL-60, K562 and U937 cells were separately co-cultured with bone marrow derived-MSCs and after 8, 16 and 24 hours, alterations in the expression of 10 chemokine genes involved in angiogenesis were evaluated by quantitative real time polymerase chain reaction (qRT PCR). Mono-cultures of leukemia cell lines were used as controls. Results: We observed that in co-culture of MSCs with HL-60 and K562 cells, the expression of CXCL10 and CXCL3 genes are increased compared to the control cells, respectively. Another part of our results was that U937 cells co-cultured with MSCs, upgrade the expression of CXCL6 gene. Moreover in U937 cells, CCL2 gene expression in the first 16 hours was lower than the control cells, while within 24 hours its expression intensified. Conclusion: Our observations for the first time have demonstrated that BM-MSCs are able to alter the expression profile of chemokine genes involved in angiogenesis, in acute myeloid leukemia cell lines. The impacts of MSCs on angiogenesis differ in various leukemia cell lines; in some cases, angiogenesis promoting, and in others, angiogenesis diminishment.
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نویسندگان مقاله | Maryam Mohammadi


| Karim Shamsasenjan


| Parvin Akbarzadehlaleh


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4924
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