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Cell Journal، جلد ۲۰، شماره ۱، صفحات ۵۳-۶۰
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عنوان انگلیسی Direct Coculture of Human Chondrocytes and Synovium-Derived Stem Cells Enhances In Vitro Chondrogenesis
چکیده انگلیسی مقاله Objective: Coculture of chondrocytes and mesenchymal stem cells (MSCs) has been developed as a strategy to overcome the dedifferentiation of chondrocytes during in vitro expansion in autologous chondrocyte transplantation. Synovium-derived stem cells (SDSCs) can be a promising cell source for coculture due to their superior chondrogenic potential to other MSCs and easy accessibility without donor site morbidity. However, studies on coculture of chondrocytes and SDSCs are very limited. The objective of this study was to investigate whether direct coculture of human chondrocytes and SDSCs could enhance chondrogenesis compared to monoculture of each cell. Materials and methods: Passage 2 chondrocytes and SDSCs were directly cocultured with various mixed ratio (3:1, 1:1, or 1:3). GAG synthetic activity was accessed with GAG assay and Safranin–O staining. Expression of chondrogenesis-related genes (collagen types I, II, X, aggrecan, and SOX-9) were analyzed by RT-qPCR and immunohistochemistry staining. Results: GAG/DNA ratio in 1:1 and 1:3 coculture groups were significantly increased compared to those in chondrocyte and SDSC monoculture groups. Type II collagen and SOX-9 were significantly upregulated in the 1:1 coculture group compared to those in chondrocyte and SDSC monoculture groups. On the other hand, osteogenic marker (type I collagen) and hypertrophic marker (type X collagen) were significantly downregulated in the coculture groups compared to those in the SDSC monoculture group. Conclusion: Direct coculture of human chondrocytes and SDSCs significantly enhanced chondrogenic potential, especially at 1:1 ratio, compared to chondrocyte or SDSC monocultures.
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نویسندگان مقاله | Tae Woo Kim


| Myung Chul Lee


| Hyun Cheol Bae


| Hyuk Soo Han


نشانی اینترنتی http://celljournal.org/journal/article/abstract/5025
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