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Cell Journal، جلد ۲۰، شماره ۱، صفحات ۱۲۰-۱۲۶
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عنوان انگلیسی Induction of Apoptosis in Cancer Cells of pre-B ALL Patients after Exposure to Platelets, Platelet-Derived Microparticles and Soluble CD40 Ligand
چکیده انگلیسی مقاله Objective: Objective: Platelets (plts) are blood cells that are involved in thrombosis, inflammation and immune responses. The in vitro treatment of tumor cells with plts, causes the inhibition of tumor cell growth although there is no agreement on the mechanism of this effect. Induction of apoptosis has been proposed as a mechanism for plt effects on tumor cells. The purpose of this study was to clarify the role of plts and plt-derived components in the induction of apoptosis in the blood mononuclear cells of patients with leukemia. Materials and methods: Materials and Methods: In this experimental study, peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood of five patients with childhood B-precursor acute lymphoblastic leukemia (pre-B ALL) and encountered with plts, plt-derived microparticles (plt-MPs) and purified soluble CD40L (sCD40L). After 48 hours of co-culture, the anti-cancer activity of the aforementioned factors was surveyed using examination of apoptosis markers of the cells including active caspase-3 and CD95 using ELISA and flow cytometry techniques, respectively. Additionally, the staining of the cells with 7-Aminoactinomycin D (7-AAD) was evaluated by flow cytometry technique. Trypan blue exclusion test and WST-1 methods were also used to compare the death/survival status of the cells. Results: Results: Levels of CD95 and caspase-3 were significantly increased in all the treatment groups (p-value< 0.05). On the other hand, trypan blue, 7-AAD and WST-1 methods showed significantly lower number of living cells in the treatment groups (p-value< 0.05). Conclusion: Conclusions: This study can show the ability of plts, plt-MPs and sCD40L for the induction of apoptosis in PBMCs of pre-B-ALL patients. Further studies are necessary to elucidate the different in vitro and in vivo effects of platelets on cancer cells.
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نویسندگان مقاله | Morteza Yaftian


| Fatemeh Yari


| Mehran Ghasemzadeh


| Vahid Fallah Azad


| Mansoureh Haghighi


نشانی اینترنتی http://celljournal.org/journal/article/abstract/5032
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