این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Cell Journal، جلد ۱۹، شماره ۴، صفحات ۵۳۷-۵۴۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی In Vitro Effects of Wistar Rat Prenatal and Postnatal Cerebrospinal Fluid on Neural Differentiation and Proliferation of Mesenchymal Stromal Cells Derived from Bone Marrow
چکیده انگلیسی مقاله Objective: Cerebrospinal fluid (CSF) plays an important role in cortical development during fetal stages. Embryonic CSF has many neurotrophic and growth factors that have been known as neurogenesis, differentiation and proliferation regulators. Mesenchymal stem cells (MSCs) are multi-potential stem cells that can differentiate into several types of mesenchymal and non-mesenchymal cells e.g. neural cells. In this study the prenatal and postnatal effects of CSF on proliferation and neural differentiation of bone marrow mesencymal stem cells (BM-MSCs) is evaluated. The study has been done on different gestational age (E19-E20) and the first day after birth (P1). Materials and methods: The mesenchymal nature of BM-MSCs proved by their adherence properties and surface markers (CD44, CD73 and CD45). In order to verify the multi-potential characteristics of BM-MSCs the osteogenic and adipogenic potential of the cells assessed. Under appropriate in vitro conditions, BM-MSCs Cultures were incubated with and without additional prenatal and postnatal CSF. MTT assay was used to quantification of cellular proliferation and viability. We have used immunocytochemistry to study the expression of MAP-2 and β-III tubulin in BM-MSCs. Length of neurites in cultured cells measured by image J software. Results: BM-MSCs differentiated into neuronal cell types when exposed to b-FGF. Viability and proliferation of BM-MSCs that conditioned with E19, E20 and P1 CSF increased in comparison with control group. Neural differentiation of BM-MSCS cultured in CSF-supplemented medium from E19 was significantly elevated compared to the control group. Conclusion: Our produced data confirmed that E19, E20 and P1 CSF are able to induce the proliferation and differentiation of BM-MSCs though they are age dependent factors. The presented data support a significant and conductive role of CSF components in neuronal survival, proliferation and differentiation
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Rozmehr Shokohi


| Mohammad Nabiuni


| Saeed Irian


| Jaleel A Miyan


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4130
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/16/article-16-851814.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات