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Cell Journal، جلد ۱۹، شماره ۴، صفحات ۵۵۹-۵۶۸
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عنوان انگلیسی Dual Effects of Cell Free Supernatants from Lactobacillus acidophilus and Lactobacillus rhamnosus GG in Regulation of MMP-9 by Up-Regulating TIMP-1 and Down-Regulating CD147 in PMA-Differentiated THP-1 Cells
چکیده انگلیسی مقاله Objective: Recent studies have reported dysregulated expression of matrix metalloproteinases (MMPs), especially MMP-2, MMP-9, tissue inhibitor of metalloproteinase-1, -2 (TIMP-1, TIMP-2), and extracellular matrix metalloproteinase inducer (CD147/EMMPRIN) in activated macrophages of patients with inflammatory diseases. Thus, MMP-2, MMP-9, and their regulators may represent a new target for treatment of inflammatory diseases. Probiotics, which are comprised of lactic acid bacteria, have the potential to modulate inflammatory responses. In this experimental study, we investigated the anti-inflammatory effect of cell-free supernatants (CFS) from two probiotics, Lactobacillus sp., L. acidophilus and L. rhamnosus GG in phorbol myristate acetate (PMA) differentiated THP-1 cells. Materials and methods: PMA-differentiated THP-1 cells were treated with CFS from L. acidophilus, L. rhamnosus and with uninoculated bacterial growth media (as a control). The expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 mRNAs were determined using real-time quantitative reverse transcription PCR. The levels of cellular surface expression of CD147 were assessed by flow cytometry, and the gelatinolytic activitiy of MMP-2 and MMP-9 were determined by zymography. Results: Our results showed that CFS from both Lactobacillus sp., L. acidophilus and L. rhamnosus GG significantly inhibited the gene expression of MMP-9 (p=0.0011 and p=0.0005, respectively), increased the expression of TIMP-1(p< 0.0001), decreased the cell surface expression of CD147 (p=0.0307 and p=0.0054, respectively), and inhibited the gelatinolytic activity of MMP-9 (p=0.0003 and p< 0.0001, respectively) in PMA-differentiated THP-1 cells. MMP-2 expression and activity and TIMP-2 expression remained unchanged. Conclusion: In conclusion, our results indicate that CFS from two probiotics, Lactobacillus sp., L. acidophilus and L. rhamnosus GG, possess anti-inflammatory properties and can modulate the inflammatory response.
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نویسندگان مقاله | Faezeh Maghsood


| Abbas Mirshafiey


| Mohadese M. Farahani


| Mohammad Hossein Modarressi


| Parvaneh Jafari


| Elahe Motevaseli


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4447
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