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Cell Journal، جلد ۱۹، شماره ۴، صفحات ۶۰۷-۶۱۳
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عنوان انگلیسی The Effect of Vitrification on Expression and Histone Marks of Igf2 and Oct4 in Blastocysts Cultured from Two-Cell Mouse Embryos
چکیده انگلیسی مقاله Objective: Vitrification is used increasingly in ART laboratories worldwide. In this study the effect of vitrification on gene expression and modifications of H3 histone in Igf2 and Oct4 genes of blastocysts cultured from non-vitrified and vitrified two-cell embryos was investigated. Materials and methods: Non–vitrified (group1) and Vitrified (group2), two-cell embryos obtained from superovulated NMRI mice were cultured in KSOM medium leading to blastocyst stage. In this experimental study, Expression of Igf2 and Oct4 and also some modifications of H3 histone in regulatory regions of these genes were compared with in vivo blastocysts as control group. In order to evaluate gene expression, Real-Time PCR and for assessment of histone modifications ChIP assay method was carried out. Results: The expression level of Igf2 in blastocysts resulted from two-cell embryos in both experimental groups was significantly higher than in vivo blastocysts. In regulatory region of Igf2 H3K9 methylation was decreased, whereas H3K9 acetylation was increased compared with the control group in experimental embryos. In contrast, the expression level of Oct4 in mentioned groups was significantly lower than in vivo blastocysts. Oct4 gene promoter demonstrated a significant increase of H3K9 methylation and decrease of H3K9 acetylation (P< 0.05). Conclusion: Our results show, both vitrification and cultivation conditions like culture media lead to some changes in expression level and modification of histone in Igf2 and Oct4 genes. These effects are the same in vitrified as non vitrified groups. Indeed in vitro culture condition is the main reason of genetic and epigenetic changes and vitrification alone could not be the main cause of the observed changes in embryos.
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نویسندگان مقاله | Maryam Jahangiri


| Maryam Shahhoseini


| Bahar Movaghar


نشانی اینترنتی http://celljournal.org/journal/article/abstract/3959
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