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Cell Journal، جلد ۱۹، شماره ۴، صفحات ۶۲۰-۶۲۶
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عنوان انگلیسی The Impact of Genetic Variation and Gene Expression Level of The Follicle-Stimulating Hormone Receptor on Ovarian Reserve
چکیده انگلیسی مقاله Objective: Ovarian reserve is the capacity of the ovary to provide fertile oocytes. Diminished Ovarian Reserve (DOR) is a disorder in which women’s ovaries are prone to go through early menopause and the loss of its function before the age of 40 results in Premature Ovarian Failure (POF) disease. Throughout folliculogenesis, follicle stimulating hormone receptor (FSHR) starts a signaling cascade in the granulosa cells which inactivating of it, arrests follicle maturation and therefore adversely affects ovarian reserve. The aim of this study was to investigate the association of polymorphisms and inactivating mutations of FSH receptor gene with POF and DOR patients. Materials and methods: A case control study was comprised of 84 POF, 52 DOR and 80 fertile Iranian women. For determining the presence of 566C>T mutation and -29G>A polymorphism in this gene, PCR-RFLP method was done. SSCP-Sequencing was used to identify any allelic variants in exon 10. The mRNA expression level of human FSHR gene was also compared between DOR and control patients, by real time-PCR. Results: Different genotypes of -29G>A and 919G>A polymorphism (exon 10) were observed, which were statistically significant. These statistically significant differences were seen in both polymorphisms when comparing the control group with only the DOR patients as well. A decrease was seen in the FSHR mRNA expression of DOR patients compared to the control group but it was not significant (P value ≥0.05). Conclusion: It can be concluded that -29G>A and 919G>A polymorphisms in the FSHR gene might be associated with diminished ovarian reserve. Although these polymorphisms at the gene level had significant differences, they might not have an effect at the FSHR expression level.
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نویسندگان مقاله | Zeinab Ghezelayagh


| Mehdi Totonchi


| Shabnam Zarei-Moradi


| Ommolbanin Asadpour


| Saman Maroufizadeh


| Poopak Eftekhari-Yazdi


| Hamid Gourabi


| Anahita Mohseni-Meybodi


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4183
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