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Cell Journal، جلد ۱۹، شماره ۴، صفحات ۶۳۴-۶۳۹
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عنوان انگلیسی Upregulation of Integrin-α6 and Integrin-β1 Gene Expressions in Mouse Spermatogonial Stem Cells after Continues and Pulsed Low Intensity Ultrasound Stimulation
چکیده انگلیسی مقاله Objective: low intensity ultrasound (continues and pulsed) is a form of energy. Spermatogonial stem cells (SSCs) maintain spermatogenesis throughout the life of the male. This study investigates the effects of low intensity ultrasound stimulation (LIUS) and Low Intensity Pulsed Ultrasound Stimulation (LIUPS) on the expression of germ cell-specific and pluripotency genes on SSCs during in vitro culture. Materials and methods: Isolated SSCs from neonatal male mice were cultured in DMEM culture medium with 10% Fetal Bovine Serum (FBS). Then to confirm identification of SSCs, PLZF protein was detected in these cells and SSCs drived colonies. SSCs were stimulated by LIUS and LIUPS for 5 days then expression of α6 and β1 integrins (germ cell-specific gene) and Oct-4 genes (pluripotency gene) were assessed at day 21th by qPCR. To investigation the proliferation rate and colonization of treated cells, counting whole number of cells and colonies and their diameters were performed with an optical microscope at every seven days. Data was analyzed by ANOVA test. Results: The LIUS and LIUPS treatment of mouse SSCs increased expression of α6 and β1 integrins genes in experimental groups compared to the control group (P< 0.05), whereas there were no significant differences between groups regarding to expression of Oct-4 gene. Also, these treatments maintained survival rate and increased proliferation rate and colonization of SSCs during first week of culture, but in next week’s proliferation rate and colonization were decreased in experimental groups. Conclusion: These results suggested that LIUS and LIUPS treatment had good effect on SSCs proliferation and colonization based on gene-specific marker expression during 21 days culture in vitro.
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نویسندگان مقاله | Mahdi Mohaqiq


| Mansoureh Movahedin


| Manijhe Mokhtari Dizchi


| Zohreh Mazaheri


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4286
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