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Cell Journal، جلد ۱۹، شماره ۴، صفحات ۶۵۴-۶۵۹
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عنوان انگلیسی Adipose Derived Stem Cells Affect miR-145 and p53 Expressions of Co-Cultured Hematopoietic Stem Cells
چکیده انگلیسی مقاله Objective: Objective: Umbilical cord blood has been used for transplantation in regenerative medicine of hematological disorders. MicroRNAs are important regulators of gene expression that control both physiological and pathological processes such as development and cancer incidence. There is a new relation between p53 (tumor suppressor gene) and miR-145 (suppressor of cell growth) up-regulation. In this study, we have assessed how adipose derived stem cells (ADSCs) affect the expansion of HSCs, miR-145 and p53 genes expression. Materials and methods: Material and methods: In the present experimental study isolated human ADSCs in passage 3 were cultured as a feeder layer. ADSCs surface markers CD73, CD90, CD105 and CD45 were detected by flow cytometry. Ex vivo cultures of cord blood CD34+ cells were performed in four culture conditions for 7 days include: CD34+ cells were cultured in medium just supplemented with cytokines, CD34+ cells were cultured on ASCs feeder layer, the cells were indirectly cultured on ADSCs feeder layer (cultured on thincert plate with 0.4 µm pore size) and the cells were analyzed immediately after extraction and considered as control group. Finally, the expressions of p53 and miR 145 genes were analyzed by real time PCR. Flow cytometry analysis of stained cells by annexin V and propidium iodide was performed for detection of apoptosis rate in expanded cells. Results: Results: Our results showed that ASCs are positive for MSCs markers CD105, CD90, CD73 and negative for HSCs markers CD34 and CD45. Our data demonstrated differentiation potential of ASCs to osteoblast cells by alizarin red and alkaline phosphatase staining. The data of MTT assay showed that proliferation rate of CD34+ cells directly cultured on a ADSCs feeder layer group were higher than other group. The direct contact between HSCs and feeder layer was prevented by microporous membrane and consequently the expression of P53 increased compared to direct contact of feeder layer with hHSCs group. The expression of p53 and miR-145 respectively was down and up-regulated significantly in HSCs cultured on ADSCs. Conclusion: It could be concluded that ADSCs may help to increase HSCs proliferation and self-renewal through miR145, p53 and their relationship.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Tahereh Foroutan


| Aisan Farhadi


| Saeed Abroun


| Bahram Mohammad Soltani


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4393
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/16/article-16-851829.pdf
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