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Cell Journal، جلد ۱۹، شماره ۴، صفحات ۶۶۰-۶۶۵
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عنوان انگلیسی Association between JMJD1A Expression and Sperm Retrieval in Non-Obstructive Azoospermic Patients
چکیده انگلیسی مقاله Objective: Identification of molecular markers which can predict the outcome of sperm retrieval in patients with non-obstructive azoospermia (NOA) will be worthwhile in the andrology clinic. JMJD1A (jumonji domain-containing 1a) is a remarkable epigenetic regulator during spermatogenesis, which plays an important role in differentiation of post-meiotic germ cells into mature spermatozoa. We aimed to consider the relationship between the JMJD1A gene expression and the outcome of sperm retrieval in patients with non-obstructive azoospermia (NOA). Materials and methods: Testicular biopsy specimens from 50 NOA patients with successful sperm retrieval (sperm+, n=22) and failed sperm retrieval (sperm−, n=28) were collected and examined for the detection of JMJD1A gene expression by quantitative real time-polymerase chain reaction (qRT-PCR) technique. Besides, conventional clinical parameters, such as luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T), age, and testicular volume, were evaluated between these two groups. Results: The data revealed that the expression of JMJD1A in the sperm+ group was significantly higher than the sperm− group (P< 0.001), whereas there were no significant differences in the clinical parameters between the two groups. The receiver operating characteristic (ROC) curve of JMJD1A expression in predicting the sperm retrieval outcome showed a sensitivity of 90.91% and a specificity of 89.29% with good discriminatory ability between the sperm+ and sperm− groups (area under the ROC curve [AUC]= 0.91). Conclusion: This study demonstrates a significant correlation between the expression of JMJD1A and the success of sperm recovery in patients with NOA, and suggests that the quantitative determination of JMJD1A mRNA in testicular biopsies can be useful for supplementing the conventional parameters in predicting the presence of spermatozoa.
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نویسندگان مقاله | Zahra Eelaminejad


| Raha Favaedi


| Tahereh Modarresi


| Marjan Sabbaghian


| Mohammad Ali Sadighi Gilani


| Maryam Shahhoseini


نشانی اینترنتی http://celljournal.org/journal/article/abstract/4409
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