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International Journal of Fertility and Sterility، جلد ۱۲، شماره ۱، صفحات ۵۱-۵۵
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عنوان انگلیسی Screening for Causative Mutations of Major Prolificacy Genes in Iranian Fat-Tailed Sheep
چکیده انگلیسی مقاله Objective: The presence of different missense mutations in sheep breeds have shown that the bone morphogenetic protein receptor 1B (BMPR1B), bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) genes play a vital role in ovulation rate and prolificacy in ewes. Therefore, the present study investigates BMPR1B, BMP15 and GDF9 genes mutations in prolific ewes of Iranian fat-tailed Lori-Bakhtiari sheep. Materials and methods: In the present experimental study, genomic DNA was extracted from whole blood of 10 prolific Lori-Bakhtiari ewes with at least two twinning records in first four parities to identify point mutations of the BMPR1B, BMP15 and GDF9 genes, using DNA sequencing. Results: The results obtained by sequencing showed a new synonymous mutation (g.66496G>A) in exon 8 of the BMPR1B gene, without any amino acid change. Sequencing of the BMP15 gene revealed a deletion of 3 bp (g.656_658delTTC) in exon 1, leading to an amino acid deletion (p.Leu19del). Four single nucleotide polymorphisms (G1:g.2118G>A, G2:g.3451T>C, G3:g.3457A>G and G4:g.3701G>A), were detected in exons 1 and 2 of the GDF9 gene, which two of them were in the origin of amino acid changes (G1: p.87Arg>His and G4: p.241Glu>Lys). These amino acid substitutions are expected to have a benign impact on structure and function of the GDF9 polypeptide sequence. Conclusion: Three major prolificacy genes (BMPR1B, BMP15 and GDF9) were polymorphic in Lori-Bakhtiari sheep, but none of the known main mutations were detected. Further studies using high throughput methods such as genome-wide association study (GWAS) and evaluation of other candidate genes are necessary for the future.
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نویسندگان مقاله | Ramin Abdoli


| Seyed Ziaeddin Mirhoseini


| Navid Ghavi Hossein-Zadeh


| Pouya Zamani


نشانی اینترنتی http://ijfs.ir/journal/article/abstract/5247
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/72/article-72-851913.pdf
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