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International Journal of Fertility and Sterility، جلد ۱۲، شماره ۱، صفحات ۶۸-۷۰
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عنوان انگلیسی Combined Effect of Retinoic Acid and Basic Fibroblast Growth Factor on Maturation of Mouse Oocyte and Subsequent Fertilization and Development
چکیده انگلیسی مقاله Objective: Many autocrine and paracrine elements that are produced within follicular niche have been the focus of much in vitro maturation research. The present study was carried out to compare retinoic acid and basic fibroblast growth factor (bFGF) efficacy on IVM of mouse oocytes, and their further dual consumption to reach an optimal protocol. Materials and methods: GV oocytes obtained from two-month-old NMRI mice were randomly divided into control, sham and 3 experimental groups. The basic culture medium was α-MEM supplemented with 10% FBS, 50 mg/l streptomycin, 60 mg/l penicillin and 10 ng/ml epidermal growth factors. Each of the experimental groups received one of the following treatments: RA (2µM), bFGF (20 ng/ml) or combination of RA and bFGF with the same concentrations. After 24 hours, capacitated spermatozoa were added to in vitro matured oocytes. Five hours later, the oocytes were cultured in fresh droplets of M2 medium for 24 hours and assessed for cleavage to the two cell stage. Results: As compared with the control group, the rate of maturation was significantly increased in the RA (p < 0.001) and bFGF+ RA (p < 0.02) groups with 58 ± 10 and 57 ± 3.46, respectively. The rate of maturation was significant in the RA (p < 0.02) and bFGF+ RA (p < 0.03) groups compared with the bFGF group. The bFGF+ RA group had a higher rate (83 ± 1.52) of two cell development than the rate in the control (33 ± 1) (P < 0.001). Conclusion: Our findings showed beneficial simultaneous effects of 2 µM RA and 20 ng/ml bFGF on mouse oocyte IVM.
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نویسندگان مقاله | Morteza Abouzaripour


| Fardin Fathi


| Erfan Daneshi


| Keywan Mortezaee


| Mohammad jafar Rezaie


| Mahdad Abdi


نشانی اینترنتی http://ijfs.ir/journal/article/abstract/5293
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/72/article-72-851916.pdf
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