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Iranian Journal of Microbiology، جلد ۳، شماره ۱، صفحات ۴۲-۴۶
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عنوان انگلیسی Fermentation, fractionation and purification of streptokinase by chemical reduction method
چکیده انگلیسی مقاله Background and Objectives : Streptokinase is used clinically as an intravenous thrombolytic agent for the treatment of acute myocardial infarction and is commonly prepared from cultures of Streptococcus equisimilis strain H46A. The objective of the present study was the production of streptokinase from strain H46A and purification by chemical reduction method. Materials and Methods: The rate of streptokinase production evaluated under the effect of changes on some fermentation factors. Moreover, due to the specific structure of streptokinase, a chemical reduction method employed for the purification of streptokinase from the fermentation broth. The H46A strain of group C streptococcus, was grown in a fermentor. The proper pH adjusted with NaOH under glucose feeding in an optimum temperature. The supernatant of the fermentation product was sterilized by filtration and concentrated by ultrafiltration. The pH of the concentrate was adjusted, cooled, and precipitated by methanol. Protein solution was reduced with dithiothreitol (DTT). Impurities settled down by aldrithiol-2 and the biological activity of supernatant containing streptokinase was determined. Results : In the fed -batch culture, the rate of streptokinase production increased over two times as compared with the batch culture and the impurities were effectively separated from streptokinase by reduction method. Conclusion : Improvements in SK production are due to a decrease in lag phase period and increase in the growth rate of logarithmic phase. The methods of purification often result in unacceptable losses of streptokinase, but the chemical reduction method give high yield of streptokinase and is easy to perform it.
کلیدواژه‌های انگلیسی مقاله Streptokinase,Fermentation,Purification,Chemical Reduction
نویسندگان مقاله z کریمی | z karimi
department of biology, faculty of science, alzahra university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

m باباشمسی | m babashamsi
department of immunochemistry, monoclonal antibody research center, avicenna research institute, acecr, tehran, iran.


e عسگرانی | e asgarani
department of biology, faculty of science, alzahra university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

m نیاکان | m niakan
department of medical microbiology,shahed university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

a سلیمی | a salimi
department of immunochemistry, monoclonal antibody research center, avicenna research institute, acecr, tehran, iran.
نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/84
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