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Iranian Journal of Microbiology، جلد ۲، شماره ۴، صفحات ۱۹۴-۱۹۷
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عنوان انگلیسی Phospholipase C in Beijing strains of Mycobacterium tuberculosis
چکیده انگلیسی مقاله Background and Objectives: Phospholipase of Mycobacterium tuberculosis plays an important role in pathogenesis through breaking up phospholipids and production of diacylglycerol. In this study, we examined the Beijing strains of Mycobacterium tuberculosis isolated from Iranian patients for the genes encoding this enzyme. Materials and Methods: DNA extraction was performed using CTAB (cetyltrimethylammonium bromide) from positive culture specimens in tuberculosis patients. PCR was then used to amplify the plcA, plcB, plcC genes of Beijing strain, and non-Beijing strains were identified by spoligotyping. Results: Of 200 specimens, 19 (9.5%) were Beijing strain and 181 (90.5%) were non-Beijing strains. The results of PCR for Beijing strains were as follows: 16 strains (84.2%) were positive for plcA, 17 (89.4%) were positive for plcB and 17 (89.4%) were positive for plcC genes. The standard strain (H37RV) was used as control. Conclusion: The majority of Beijing strains have phospholipase C genes which can contribute to their pathogenesis but we need complementary studies to confirm the role of phospholipase C in pathogenecity of Mycobacterium tuberculosis.
کلیدواژه‌های انگلیسی مقاله Mycobacterium tuberculosis,Beijing strains,phospholipase C
نویسندگان مقاله هادی پور گودرزی | h goudarzi
department of microbiology, faculty of medicine, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

es میرصمدی | es mirsamadi
department of microbiology, faculty of medicine, shahid beheshti university of medical sciences, tehran, iran and mycobacterium research center mrc national research institute of tuberculosis and lung disease nritld , tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

s جهانی شرافت | s jahani sherafat
department of microbiology, faculty of medicine, shahid beheshti university of medical sciences, tehran, iran and mycobacterium research center mrc national research institute of tuberculosis and lung disease nritld , tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

مسعود اصفهانی | m esfahani
school of medicine, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

n فرامرزی | n faramarzi
school of medicine, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

p فرنیا | p farnia
mycobacterium research center mrc national research institute of tuberculosis and lung disease nritld , tehran, iran.
نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/71
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