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Iranian Journal of Microbiology، جلد ۲، شماره ۲، صفحات ۷۳-۷۹
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عنوان انگلیسی Direct detection and serogroup characterization of Neisseria meningitidis from outbreak of meningococcal meningitis in Delhi
چکیده انگلیسی مقاله Background and Objectives: Rapid clinical manifestation/progression of the meningococcal meningitis and lacunae in conventional bacteriological test often encourages indiscriminate use of antibiotics much before the etiology is established. This study was planned to evaluate ctrA PCR for rapid molecular detection. In addition, multiplex PCR and sequencing was done for serogroup prediction to provide essential epidemiological and laboratory evidence for decision makers of health department of the country for choosing appropriate vaccine and phylogenetic analysis to establish lineage. Materials and Methods : 73 CSF samples, collected from equal number of suspected cases, were investigated by both bacteriological (microscopy, culture, LA and drug sensitivity testing) as well as molecular tests i.e. PCR targeting conserved ctrA gene, multiplex PCR for serogroup characterization and DNA sequencing. Results : ctrA PCR revealed sensitivity, specificity, positive predictive value and negative predictive values of 93.15%, 100%,100%, and 88.23% respectively. Multiplex PCR based genogrouping followed by DNA sequencing, BLAST and phylogenetic analysis revealed complete homology with earlier submitted Neisseria meningitidis serogroup A strain Z2491 to suggest the sole involvement of only serogroup A in the outbreak. Two strains showed resistance to cefuroxime, ciprofloxacin, nalidixic acid. Only one strain showed resistance to ciprofloxacin, emphasizing the need for a constant surveillance system. Conclusion : These diagnostic molecular tools are of paramount importance in establishing etiology, serogrouping, and epidemiological surveillance especially in developing countries like India.
کلیدواژه‌های انگلیسی مقاله Neisseria meningitidis,ctr A PCR,myn B gene
نویسندگان مقاله ss negi | ss negi
biotechnology amp;amp; biochemistry divisiondirectorate, national centre for disease control ncdc previously nicd , 22-shamnath marg, delhi-54, india.


ss grover | ss grover
biotechnology amp;amp; biochemistry divisiondirectorate, national centre for disease control ncdc previously nicd , 22-shamnath marg, delhi-54, india.


ss rautela | ss rautela
biotechnology amp;amp; biochemistry divisiondirectorate, national centre for disease control ncdc previously nicd , 22-shamnath marg, delhi-54, india.


ds rawat | ds rawat
biotechnology amp;amp; biochemistry divisiondirectorate, national centre for disease control ncdc previously nicd , 22-shamnath marg, delhi-54, india.


s گوپتا | s gupta
microbiology division national centre for disease control ncdc previously nicd , 22-shamnath marg, delhi-54, india.


s خواره | s khare
national centre for disease control ncdc previously nicd , 22-shamnath marg, delhi-54, india.


s لعل | s lal
national centre for disease control ncdc previously nicd , 22-shamnath marg, delhi-54, india.


a رای | a rai
biotechnology amp;amp; biochemistry divisiondirectorate, national centre for disease control ncdc previously nicd , 22-shamnath marg, delhi-54, india.
نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/50
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