این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۱، شماره ۴، صفحات ۹-۱۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Detection of colon flora in peritoneal drain fluid after colorectal surgery: can RT-PCR play a role in diagnosing anastomotic leakage?
چکیده انگلیسی مقاله Background and objectives: A semi-quantitative Real-Time PCR strategy was developed to identify indicator organisms for an indication on anastomotic leakage in peritoneal drainage fluid, Escherichia coli and Enterococcus faecalis. Materials and Methods: The analytical performance of the amplification method was validated with 10 culture-positive and 7 culture negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis. Results: Real-Time PCR results were fully concordant with the microbiological culture results. However, among the culture negative samples, four false-positive RT-PCR results were found. All false-positives originated from a single patient with a surgical site infection. This may indicate an elevated sensitivity of the RT-PCR method. Conclusion: The results showed that the semi-quantitative RT-PCR method has the clear potential to be useful as a powerful tool in early detection of anastomotic leakage.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله ویلم b وان leeuwen | willem b van leeuwen
department of surgery, erasmus medical center rotterdam, rotterdam, the netherlands and toplab, leiden university of applied science, niels bohrweg 11-13, leiden, the netherlands.


n komen | n komen
department of surgery, erasmus medical center rotterdam, rotterdam, the netherlands and department of surgery, zna middelheim, antwerp, belgium.


mc morsink | mc morsink
toplab, leiden university of applied science, niels bohrweg 11-13, leiden, the netherlands.


s beiboer | s beiboer
toplab, leiden university of applied science, niels bohrweg 11-13, leiden, the netherlands.


a miggelbrink | a miggelbrink
toplab, leiden university of applied science, niels bohrweg 11-13, leiden, the netherlands.


p willemsen | p willemsen
department of surgery, zna middelheim, antwerp, belgium.


e وان درخشش harst | e van der harst
department of surgery, maasstad ziekenhuis rotterdam, rotterdam, the netherlands.


jf lange | jf lange
department of surgery, erasmus medical center rotterdam, rotterdam, the netherlands.
نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/32
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات