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Iranian Journal of Microbiology، جلد ۱، شماره ۱، صفحات ۱۳-۲۱
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عنوان انگلیسی Multiple- mutations in the katG gene of Mycobacterium tuberculosis isolates correlate with high- level of resistance to isoniazid in patients with active pulmonary tuberculosis from Belarus
چکیده انگلیسی مقاله Background and Objectives: The aim of this study was to investigate the significance of multiple-mutations in the katG gene, predominant nucleotide changes and its correlation with high level of resistance to isoniazid in Mycobacterium tuberculosis isolates that were randomly collected from sputa of 42 patients with primary and secondary active pulmonary tuberculosis from different geographic regions of Belarus. Materials and Methods: Drug susceptibility testing was determined using the CDC standard conventional proportional method. DNA extraction, katG amplification, and DNA sequencing analysis were performed. Results: Thirty four (80%) isolates were found to have multiple-mutations (composed of 2-5 mutations) in the katG. Increased number of predominant mutations and nucleotide changes were demonstrated in codons 315 (AGC→ACC) , 316 (GGC→AGC) , 309 (GGT→GTT) with a higher frequency among patients bearing secondary tuberculosis infection with elevated levels of resistance to isoniazid (MIC μg/ml ≥ 5-10) . Furthermore it was demonstrated that the combination of mutations with their predominant nucleotide changes were also observed in codons 315, 316, and 309 indicating higher frequencies of mutations among patients with secondary infection respectively. Conclusion: In this study 62% (n=21) of multi-mutated isolates found to have combination of mutations with predominant nucleotide changes in codons 315 (AGC→ACC) , 316 (GGC→GTT) , 309 (GGT→GGT) , and also demonstrated to be more frequent in isolates of patients with secondary infections, bearing higher level of resistance to isoniazid (≥ 5 -10μg/ml).
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نویسندگان مقاله سعید ذاکر بستان آباد | saeed zakerbostanabad
department of clinical microbiology, belarusian research institute for epidemiology and microbiology, minsk, belarus and deptartment of mycobacteriology and pulmonary research; pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

v ملا کاظمی | v molla kazemi
department of microbiology, faculty of medicine, islamic azad university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

mk رحیمی | mk rahimi
cell bank of iran, pasteur institute of iran,tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

شهیدی sh شهیدی | shahidi sh shahidi
deptartment of mycobacteriology and pulmonary research; pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

sh پورآذر | sh pourazar
department of clinical microbiology, belarusian research institute for epidemiology and microbiology, minsk, belarus.


m معصومی | m massomi
deptartment of mycobacteriology and pulmonary research; pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

a نور نعمت الهی | a nour nemattolahi
deptartment of mycobacteriology and pulmonary research; pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

f عبدالرحیمی | f abdolrahimi
deptartment of mycobacteriology and pulmonary research; pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

lk surkova | lk surkova
tuberculosis and pulmonary research institute, minsk, belarus.


m غضنفری | m ghazanfari
deptartment of mycobacteriology and pulmonary research; pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

lp تیتوف | lp titov
department of clinical microbiology, belarusian research institute for epidemiology and microbiology, minsk, belarus.
نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/3
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