این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Family and Reproductive Health، جلد ۳، شماره ۲، صفحات ۵۱-۵۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Application of Molecular Cytogenetic Technique for Rapid Prenatal Diagnosis of Aneuploidies in Iranian Population
چکیده انگلیسی مقاله Objective: Classic cell culture and karyotyping is routinely used for prenatal detection of different chromosomal abnormalities. Molecular cytogenetic techniques have also recently been developed and used for this purpose. Quantitative florescence PCR using short tandem repeat (STR) markers has more potential for high throughput diagnosis. Marker heterozygosity in short tandem repeats (STR) is of critical importance in the clinical applicablity of this method. Materials and Methods: Different STR markers on chromosomes 13, 18, 21, X and Y were analysed from amniotic samples to detect related disorders such as Down, Edward, Patau, Klinefelter sundromes , as well as sex chromosomes numerical abnormalities . Results: In our population some markers (D18S976, DXS6854, D21S11, and D21S1411) showed alleles with sizes out of expected ranges. But others occupied narrower range of predicted distribution. Most markers have enough heterozygosity (66.3-94.7) to be used for prenatal diagnosis. Furthermore, results obtained from full karyotype for all samples were in concordance with results of molecular cytogenetic testing. Conclusion: It is concluded that, in urgent situations, if proper markers used, molecular cytogenetic testing (QF-PCR) could be a useful method for rapid prenatal diagnosis (PND) in populations with high rate of consanguinity such as Iran.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله حبیب نصیری | habib nasiri
department of medical genetics, school of medicine, tehran university of medical sciences, tehran, i

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

ژیلا داستان | jila dastan
iranian fetal medicine foundation, tehran, iran


محمد حسن سیفی | mohammad hasan seifi
school of medicine, iran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

نوری دلویی | noori dalooi
department of medical genetics, school of medicine, tehran university of medical sciences, tehran, i

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

سعید رضا غفاری | saeed reza ghaffari
department of medical genetics, school of medicine, tehran university of medical sciences, tehran, i

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://jfrh.tums.ac.ir/index.php/jfrh/article/view/66
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات