این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Pathology، جلد ۱۱، شماره ۴، صفحات ۳۵۴-۳۶۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Isolation of indigenous Glutathione producing Saccharomyces cerevisiae strains
چکیده انگلیسی مقاله Background: Glutathione (GSH) is a non-protein thiol compound, which plays an important role in the response to oxidative stress and nutritional stress. The aim of this study was to isolate indigenous S. cerevisiae strains capable of effectively produce GSH. Methods: One hundred-twenty sweet fruit samples were collected. The strains were isolated on yeast glucose chloramphenicol (YGC) agar medium and identified. The isolates were evaluated for GSH producing on yeast malt (YM) medium. Concentration of glutathione was investigated by recording absorbance of all samples at wavelength 412 nm (Ellman’s method). In addition, optimization of glucose and peptone concentration in culture medium and the effects of various environmental conditions such as temperature (20–35 °C), agitation rate (150–250 rpm), and initial pH (4.0–6.0) were assessed on producing of GSH. Results: From 120 samples, 80 isolates were identified by morphological, biochemical and molecular tests as S. cerevisiae. Five isolates were capable to produce effectively GSH. The optimal culture conditions were agitation rate, 200 rpm; temperature, 30 °C; initial pH, 6; glucose, 30 g/l; and peptone concentration, 5 g/l. In optimal conditions, the amount of derived glutathione was improved compared to YM basal medium and highest GSH concentration (296.8 mg/l) was obtained after cultivation with shaking for 72 h. Conclusion: The possibility of obtaining S. cerevisiae cells with a high GSH intracellular content can be considered an interesting opportunity of furthering the range of application and utilization of this molecule.
کلیدواژه‌های انگلیسی مقاله Glutathione,Saccharomyces cerevisiae,yeast
نویسندگان مقاله طاهره طهماسبی |
dept. of microbiology, faculty of advanced science amp; technology, pharmaceutical science branch, islamic azad university, tehran, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

رحیم نصرتی |
dept. of pharmaceutical biotechnology, school of pharmacy, mashhad university of medical sciences, mashhad, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم دارویی (Islamic azad university pharmaceutical sciences branch)

حامد زارع |
dept. of pharmaceutical biotechnology, school of pharmacy, shahid beheshti university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

حوریه صادری |
molecular microbiology research center, shahed university, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

ریحانه مرادی |
dept. of microbiology, faculty of advanced science amp; technology, pharmaceutical science branch, islamic azad university, tehran, iran
سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

پرویز اولیا |
molecular microbiology research center, shahed university, tehran, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم دارویی (Islamic azad university pharmaceutical sciences branch)

نشانی اینترنتی http://ijp.iranpath.org/article_21545_3dd717d3d960a1ab362e8812c9a30b96.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده 1
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات