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Iranian Journal of Pathology، جلد ۱۱، شماره ۲، صفحات ۱۳۸-۱۴۳
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عنوان انگلیسی Molecular Detection of Ureaplasma urealyticum from Prostate Tissues using PCR-RFLP, Tehran, Iran
چکیده انگلیسی مقاله Background: In most cases, prostatitis can be caused by a bacterial agent such as Ureaplasma urealyticum. Considering to the cumbersome of the culture method for the detection of Ureaplasma species in clinical samples such as prostate; PCR method that is faster and more appropriate than the cultivation methods, can be utilized for the detection of U. urealyticum and U. parvum. PCR-RFLP method can differentiate both biovars and assist in studies of the clinical diagnosis, epidemiology and pathology of this species in human. The aim of this study was to molecular detection of U. urealyticumin in prostate tissue samples based on PCR- RFLP. Methods:Two hundred prostate tissue samples were collected from patient suffering from prostatitis. The PCR assay was used to amplify a 559 bp fragment of 16S-23SRNA interspace region of Ureaplasma. After sequencing, PCR products from positive samples were digested with TaqI restriction enzyme. Results: Seven cases (3.5%) out of 200 prostate tissue samples were positive for U. urealyticum. Results of PCR products sequencing demonstrated that all isolates were U. parvum biovar. PCR-RFLP results shown that there was not any differentiation in pattern of enzymatic digestion, in addition, all isolates were U. parvum, serovar 3. Conclusion: U. urealyticum can be one of the causing agents of prostatitis. Using PCR-RFLP with specific primer and restriction enzyme is a rapid and cost-effect method for detection and differentiation of Ureaplasma from clinical samples.
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نویسندگان مقاله غلامرضا ایراجیان |
dept. of microbiology, iran university of medical science, tehran, iran

مهری شریفی |
dept. of microbiology, islamic azad university, zanjan branch, zanjan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

شیوا میرکلانتری |
dept. of microbiology, semnan university of medical sciences, semnan, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی زنجان (Islamic azad university of zanjan)

رضا میرنژاد |
molecular biology research center, baqiyatallah university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی سمنان (Semnan university of medical sciences)

محمدرضا جلالی ندوشن | mohammad reza jalali nadoushan
dept. of pathology, school of medicine, shahed university, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

نفیسه قربانپور | nafiseh ghorbanpour
dept. of microbiology, islamic azad university, zanjan branch, zanjan, iran.

سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

نشانی اینترنتی http://ijp.iranpath.org/article_19275_f2c5c2653487c9b8c1f4dc4160adeaaa.pdf
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