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Iranian Journal of Pathology، جلد ۶، شماره ۴، صفحات ۱۷۳-۱۷۸
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عنوان انگلیسی Gene Expression under F8 Promoter Driving In Mouse Hepatoma Cells: A Step towards Gene Therapy of Hemophilia
چکیده انگلیسی مقاله Background and Objectives: Significant progress has been made in treatment of hemophilia. Ex-vivo gene therapy is going popular due to the capability of this method in using isogenic cells for genetic manipulation and reintroducing them into same host after proliferation. Most gene therapy techniques use viral vectors, which usually harbor a strong and non-specific promoter (e.g. CMV early promoter) for driving the downstream gene. This may be a disadvantage due to uncontrollable nature of gene expression. In addition, considering the potentials of recently introduced stem cells as reservoirs and their potential to differentiate to other cell lines, uncontrolled expression may have unknown outcomes. To make gene therapy of hemophilia more resembling to the nature, we supposed F8 promoter might be a good candidate for driving downstream F8 coding sequence. Materials and Methods: To test our hypothesis, we designed and constructed a DNA construct by PCR, which harbors EGFP coding sequence downstream to mouse F8 promoter and transfected it to a mouse hepatoma cell line. Transfection was assayed qualitatively by fluorescent microscopy. Results: Fluorescence was detected in transfected cells a sign of presence of EGFP. Conclusion: F8 promoter can drive expression of downstream genes, a capability which and may have potential to be used in gene therapy of hemophilia. A conclusion that should be examined by further studies.
کلیدواژه‌های انگلیسی مقاله Gene therapy,Hemophilia,Hepatoma cells
نویسندگان مقاله پژمان فرد اصفهانی | pejman fard esfahani
dept. of biochemistry, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

مهدی کدیور | mehdi kadivar
dept. of biochemistry, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

مژگان الهیاری | mojgan allahyari
dept. of education, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

فاطمه میرخانی | fatemeh mirkhani
dept. of education, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

نشانی اینترنتی http://ijp.iranpath.org/article_8408_1015690b766deeb79d93232dacf5cca1.pdf
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