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Iranian Journal of Pathology، جلد ۴، شماره ۴، صفحات ۱۷۷-۱۸۱
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عنوان انگلیسی Using a Temperature Gradient against the Time in Polyacrylamide Gel Electrophoresis May Eliminate the Need for Stacking Gels
چکیده انگلیسی مقاله Background and Objectives: Making stacking gels for polyacrylamide gels in the laboratory by conventional methods is laborious and time consuming. Considering the role of temperature in polyacrylamide gels with respect to electrical resistance and viscosity, we assumed that decreasing the temperature would cause an increase in electrical resistance and viscosity. Ultimately, a downward temperature gradient imposed in the first phase of polyacrylamide gel running time would supposedly improve the migration of macromolecules. This project analyzed the effect of temperature gradient on the migration of macromolecules in the continuous gels (without stacking) and compared it with results obtained using stacking gels. Material and Methods: Electrical resistance was calculated using Ohm's law. Subsequently, to examine the effect of temperature change on macromolecules separation, conformation sensitive gel electrophoresis (CSGE) was used as a model and specimens were run under three different conditions, one of which was prepared with a stacking gel. Results: The electrical resistance showed an inverse relationship with temperature in this study. Separation of the DNA molecules in the continuous gels (with no stacking) was comparable with the conventional method (with stacking). Conclusion: Using a temperature gradient against time may be an alternative method for stacking gels.
کلیدواژه‌های انگلیسی مقاله temperature,Polyacrylamide Gel Electrophoresis
نویسندگان مقاله پژمان فرد اصفهانی | fard esfahani
dept. of biochemistry, pasteur institute of iran, tehran, iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

پیمان محمدی تربتی | mohammadi torbati
dept. of pathology, shahid beheshti university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

نشانی اینترنتی http://ijp.iranpath.org/article_8854_ffada29126f82bf86c7f455c77991f12.pdf
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