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Iranian Journal of Pediatric Hematology and Oncology، جلد ۶، شماره ۱، صفحات ۴۳-۵۱
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عنوان انگلیسی Candidiasis in Pediatrics; Identification and In vitro Antifungal Susceptibility of the Clinical Isolates
چکیده انگلیسی مقاله Background Candida species are normal microflora of oral cavity, vagina, and gastrointestinal tract. They are the third most prevalent cause of pediatric health care–associated bloodstream fungal infection. This study aimed to provide an epidemiological feature of candidiasis and also presents an antifungal susceptibility profile of clinical Candida isolates among children. Materials and Methods During July 2013 to February 2015, 105 patients from different hospitals of Isfahan, Iran, were examined for candidiasis by phenotypic tests. Samples were obtained from nail clippings, blood, thrush, BAL, urine, oropharynx, skin, and eye discharge. The age range of patients was between 18 days to 16 years. Genomic DNA of isolates was extracted and ITS1- 5.8SrDNA-ITS2 region was amplified by ITS1 and ITS2 primers. The PCR products were digested using the restriction enzyme MspI. Minimum inhibitory concentration (MICs) was determined using microdilution broth method according to the clinical and laboratory standards institute (CLSI) M27-A3 and M27-S4 documents. Results Forty-three patients (40.9%) had Candida infection.The most clinical strains were isolated from nail infections (39.5%), and candidemia (13.9%). Candida albicans was the most prevalent species (46.5%). MICs ranges for amphotericin B, fluconazole, and itraconazole were (0.025- 0.75 μg/ml), (0.125-16 μg/ml), and (0.094- 2 μg/ml), respectively. Conclusion Due to high incidence of Candida infections among children, increasing of fatal infection like candidemia, and emersion of antifungal resistance Candida isolates, early and precise identification of the Candida species and determination of antifungal susceptibility patterns of clinical isolates may lead to better management of the infection.
کلیدواژه‌های انگلیسی مقاله Antifungal susceptibility, Candidemia, Pediatrics
نویسندگان مقاله r محمدی | r mohammadi
department of medical parasitology and mycology, school of medicine, isfahan university of medical sciences, isfahan, i

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)
سازمان های دیگر: Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

b عطایی | b ataei
infectious diseases and tropical medicine research center, isfahan university of medical sciences, isfahan, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

نشانی اینترنتی http://ijpho.ssu.ac.ir/browse.php?a_code=A-10-70-73&slc_lang=en&sid=en
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