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Medical Journal of Islamic Republic of Iran، جلد ۱۵، شماره ۲، صفحات ۱۰۳-۱۰۸
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عنوان انگلیسی PREPARATION OF HIGHLY PURIFIED SOLVENTDETERGENT COA GULATION FACTOR VII AND FACTOR IX CONCENTRATES FROM PROTHROMBIN COMPLEX (PPSB)
چکیده انگلیسی مقاله In this study, anion-exchange chromatography was used to purify factor VII and factor IX from prothrombin complex (PPSB), which contains coagulation factors II, VII, IX and X. For this purpose, DEAE-Sepharose CL-6B gel , Pharmacia column XK-26 , high flow rate and two stepwise gradients with phosphate citrate buffer were used. The yield of the two lyophylized products, factor VII and factor IX concentrates, was 61 % and 75%, respectively. Specific activity of factor VII increased from 0.16 to 3.06 (purification-fold= 19.1) and specific activity of factor IX increased from 12 to 4.9 (purification-fold= 4.1). Results of electrophoresis on agarose gel-as well as immunoelectrophoresis-indicated higher purity of factor VII and factor IX compared to PPSB. Thrombogenicity of the two products were within the normal range defined for PPSB. In order to improve viral safety, solvent-detergent treatment was performed prior to further purification. Factor VII concentrate is used for patients with factor VII deficiency and also for hemophilia patients with inhibitors. Factor IX concentrate is used for treatment of hemophilia B patients.
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نویسندگان مقاله s نصیری | s nasiri
from the department of research and development, blood research and fractionation co., tehran,


h رضوان | h rezvan
from the department of research and development, blood research and fractionation co., tehran,

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

k موسوی | k mousavi
from the department of research and development, blood research and fractionation co., tehran,

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

mh روستایی | mh roostaei
department of virology, tarbiat modarres university, tehran, f.r. fran

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-266&slc_lang=en&sid=en
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