| چکیده انگلیسی مقاله |
It is now agreed that 10-25% of infertile men with azoospermia have submicroscopic deletions of the Y chromosome long ann (yq), consistent with the proposed location of the azoospermia locus (AZF) in Yq 11.23. However, it is not known whether Yq microdeletions are unique to men with azoospermia or whether they are also observed in infertile men with less severe defects of spermatogenesis (oligozoospermia). The objective of this study was to determine the prevalence of Yq deletions in infertile men with idiopathic oligozoospermia DN A was extracted from blood lymphocytes of 45 oligozoospermic men (sperm densities < 20 million/mL) in whom known causes of infertility had been excluded. All subjects were typed for the 27 Y -specific STSs that have been mapped to deletion interval 6. An STS was considered negative if no PCR product was observed in 3 reactions, in which a fertile male gave a specific PCR product and anorma1 female DNA did not. Whenever sufficient DNA was available, deletions detected by PCR were verifed by Southern hybridization. Of the 45 oligozoospermic men, 4 (9010) had deletions of one or more STSs. These deletions were verified by Southern hybridization. All. 4 deletions were located in distal interval 6 (6C and 6D) and included the DAZ (Deleted in Azoospermia) gene, a Y-specific gene that has been proposed as a candidate for male infertility. All four patients had sperm densities of less than 1 million/mL and three out of these 4 patients had mean testis volume of less than 15 mL. Two of the infertile men with Y deletions had a testicular biopsy testicular histology in both of these patients was consistent with germ cell arrest, spermatocyte stage. Yq microdeletions are not unique to infertile men with azoospermia but are abserved also in infertile men with oligozoospermia Taken together with previously reported studies on azoospermic men, these results indicate that Yq deletions are predominantly obselVed in, but are not limited to, infertile men with relatively severe defects of spermatogenesis. |
| نویسندگان مقاله |
h نجم آبادی | h najmabadi
from the welfare science and rehabilitation university hn , tehran, iran; karimi-nejad pathology amp; genetic center hn, ss, fs , tehran, iran; division of endocrinology, metabolism, and molecularmedicine kk. wt, lr, an, mg, sb and department of obstetrics and gynecology bs , charles r.drew university of medicine and science, los angeles, ca, usa; department of obstetrics andgynecology, university of melbourne, australia; institute of reproduction and development dmdk,rim. kal , monash university, melbourne, australia, and prince henry s medical research institute rimc , and karolinska institute, stokholm, sweden s a .
dm de kretser | dm de kretser
division 0 endocrinology, metabolism, and molecular medicine kk. wt, lr, an, mg, sb and department o obstetrics and g
سازمان اصلی تایید شده: دانشگاه علوم بهزیستی و توانبخشی (University of social welfare and rehabilitation sciences)
s arver | s arver
department o obstetrics and gynecology, university 0 melbourne, australia; institute 0 reproduction and development d
سازمان اصلی تایید شده: 0 دانشگاه های خارج از کشور
w تایلر | w taylor
department o obstetrics and gynecology, university 0 melbourne, australia; institute 0 reproduction and development d
سازمان اصلی تایید شده: 0 دانشگاه های خارج از کشور
c mallidis | c mallidis
department o obstetrics and gynecology, university 0 melbourne, australia; institute 0 reproduction and development d
سازمان اصلی تایید شده: 0 دانشگاه های خارج از کشور
hw gorden بکر | hw gorden baker
department o obstetrics and gynecology, university 0 melbourne, australia; institute 0 reproduction and development d
سازمان اصلی تایید شده: 0 دانشگاه های خارج از کشور
ri mclachlan | ri mclachlan
department o obstetrics and gynecology, university 0 melbourne, australia; institute 0 reproduction and development d
سازمان اصلی تایید شده: 0 دانشگاه های خارج از کشور
ka loveland | ka loveland
department o obstetrics and gynecology, university 0 melbourne, australia; institute 0 reproduction and development d
سازمان اصلی تایید شده: 0 دانشگاه های خارج از کشور
l ramirez | l ramirez
department o obstetrics and gynecology, university 0 melbourne, australia; institute 0 reproduction and development d
سازمان اصلی تایید شده: 0 دانشگاه های خارج از کشور
a nieva | a nieva
سازمان اصلی تایید شده: 0 موسسات و مراکز خارج از کشور
m gutierrez | m gutierrez
department o obstetrics and gynecology, university 0 melbourne, australia; institute 0 reproduction and development d
سازمان اصلی تایید شده: 0 دانشگاه های خارج از کشور
| s sahebjam
department o obstetrics and gynecology, university 0 melbourne, australia; institute 0 reproduction and development d
سازمان اصلی تایید شده: 0 دانشگاه های خارج از کشور
| s sahebjam
department o obstetrics and gynecology, university 0 melbourne, australia; institute 0 reproduction and development d
سازمان اصلی تایید شده: 0 دانشگاه های خارج از کشور
f صاحب جم | f sahebjam
department o obstetrics and gynecology, university 0 melbourne, australia; institute 0 reproduction and development d
سازمان اصلی تایید شده: 0 دانشگاه های خارج از کشور
s bhasin | s bhasin
department o obstetrics and gynecology, university 0 melbourne, australia; institute 0 reproduction and development d
سازمان اصلی تایید شده: 0 دانشگاه های خارج از کشور
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