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Journal of Medical Bacteriology، جلد ۳، شماره ۳-۴، صفحات ۸-۱۳
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عنوان انگلیسی A New Purification Method for Beta-Toxin of Clostridium perfringens Type C Vaccinal Strain
چکیده انگلیسی مقاله Background : Clostridium perfringens type C strains cause severe necrotizing enteritis in sheep, calves, goats, pigs and humans. Beta-toxin is introduced to be the essential virulence factor of this microorganism. In the present study, a new method was established for the purification of beta toxin from culture supernatant fluid of C. perfringens type C vaccinal strain. Methods : The four steps of the purification scheme involved ammonium sulfate precipitation, cation exchange chromatography (CM- Sepharose), anoion exchange chromatography (DEAECellulose) and gel filtration (Sephadex G-100). Results : Beta toxin was purified about 78-fold from the Sephadex G-100 column with a yield of about 16.7% in terms of lethality of the toxin. The molecular weight of the beta toxin as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was approximately 37 KD. The LD50 for adult mice was 2.21 μg/kg. Human umbilical vein endothelial cells (HUVEC) exposed to CPB showed a cell border retraction, cytoplasmic blebbing, cell shrinkage and cell rounding. The toxin was heat labile and was inactivated by trypsin. Conclusions : In conclusion, the results of this study showed the new protocol is suitable for purification of beta toxin of C. perfringens type C regarding good purity, good yields and high activity of beta toxin.
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نویسندگان مقاله احسان زایرزاده | ehsan zayerzadeh
department of biology, faculty of food industry and agriculture, standard research institute, karaj, ir iran.


آزاده فردی پور | azadeh fardipour
department of vaccine quality control, razi vaccine and serum research institute, karaj, ir iran.

سازمان اصلی تایید شده: موسسه تحقیقات واکسن و سرم سازی رازی (Razi vaccine and serum research institute)

احمدرضا جباری | ahmadreza jabbari
department of anaerobic bacterial vaccine research amp;amp; production, razi vaccine and serum research institute, karaj, ir iran.

سازمان اصلی تایید شده: موسسه تحقیقات واکسن و سرم سازی رازی (Razi vaccine and serum research institute)

نشانی اینترنتی http://jmb.tums.ac.ir/index.php/jmb/article/view/25
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Articles
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