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Journal of Medical Bacteriology، جلد ۱، شماره ۳-۴، صفحات ۱۰-۱۶
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عنوان انگلیسی A New Approach for Designing a Potentially Vaccine Candidate against Urinary Tract Infection by Using Protein Display on Lactobacillus
چکیده انگلیسی مقاله B ackground: The prevalence of Urinary Tract Infection (UTI) is really high in the world. Escherichia coli is a major agent of UTI. One of the strategies for decreasing UTI infections is vaccine development. As the attachment is a really important stage in colonization and infection, at- tachment inhibition has an applied strategy. FimH protein is a major factor during bacterial colonization in urinary tract and could be used as a vaccine. Thus, it was considered in this research as a candidate anti- gen. Methods: The sequences of fimH and acmA genes were used for de- signing a synthetic gene. It was cloned to pET23a expression vector and transformed to E. coli (DE3) Origami. To confirm the expression of recombinant protein, SDS-PAGE and western blotting methods were used. Subsequently, recombinant protein was purified. On the other hand, Lactobacillus reuteri was cultured and mixed with FimH / AcmA recombinant protein. The rate of protein localization on lactobacillus surface was assessed using ELISA method. R esults: It was showed that the recombinant protein was expressed in E. coli (DE3) Origami and purified by affinity chromatography. More- over, this protein could be localized on lactobacillus surface by 5 days. Conclusion: In current study, a fusion recombinant protein was pre- pared and displayed on L. reuteri surface. This strain could be used for animal experiment as a competitor against Uropathogenic E. coli (UPEC). Using manipulated probiotics strains instead of antibiotic ther- apy could decrease the antibiotic consumption and reduce multi-drug resistant strains.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله دکتر غلامرضا گودرزی | gholamreza goudarzi
department of microbiology, faculty of medicine, lorestan university of medical sciences, khorramabad, ir iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی لرستان (Lorestan university of medical sciences)

جلیل فلاح مهرآبادی | jalil fallah mehrabadi
the lister institute of microbiology, tehran, ir iran.


فاطمه فراهانی حاتم آبادی | fatemeh farahani hatamabadi
department of microbiology, science and research branch, islamic azad university, tehran, ir iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

هدروشا ملا آقا میرزایی | hedroosha molla agha mirzaei
the lister institute of microbiology, tehran, ir iran.


سارا نجفی | sarah najafi
pharmaceutical incubator center, tehran university of medical sciences, tehran, ir iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://jmb.tums.ac.ir/index.php/jmb/article/view/18
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Articles
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