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Medical Journal of Islamic Republic of Iran، جلد ۱۴، شماره ۱، صفحات ۵۳-۵۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی A NEW CYTOCOLORIMETRIC ASSAY USING PHOSPHATAS E ACTIVI T Y FOR MEASURING CELLULAR FUNCTIONS
چکیده انگلیسی مقاله In this study cellular phosphatase activity, referred to as enzyme developed color (EDC) assay, was used for an in vitro assessment of cell proliferation and cytotoxicity. Optimum conditions were established and found to be 10 x103 cells / well at 37°C and 60 min incubation (for developing color). Under the same conditions a direct correlation between optical density (00) and cell number was observed. Thus, the ODs for a cell line, Ish at 100, 50, 25 and 10 X 103 cells/well were 1.0S ±0.05, 0.59 ±0.02, 0.33 ±0.02, and 0.17 ±0.01 respectively (r=0.995,p< 0.001). When EDC was compared with another colorimetric assay, i.e. MTT, the results showed a direct correlation with r=0.995, p< 0.001 . Repeating the experiment with live and fixed tumor cells showed similar results. Thus, for the Ish line, the ODs at 50 and 10 X 103 cells/well for fixed and live cells were 0.66±0.01, 0.18±0.01 and O.64±0.03 (p>0.05), 0.19±0.01 (p>0.05), respectively. The assay was also shown to be suitable for the measurement of cell cytotoxicity and compared well with the MTT assay. These findings indicated that a simple, rapid and economical EDC assay could be used to investigate various cellular functions. The main advantage of EDC is it's suitability to use stored cells. This provides flexibility for testing samples stored over a long period in order to limit inter-experimental variations
کلیدواژه‌های انگلیسی مقاله Alkaline phosphatases, testis tumor, cell proliferation
نویسندگان مقاله a a n p m dabare | a a n p m dabare
from the department of medical oncology, the royal london hospital, whitechapel, london, e1 1bb, u.k.


a m e نوری | a m e nouri


mrc path | mrc path


r t d الیور | r t d oliver


نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-354&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Oncology
نوع مقاله منتشر شده Original Research: Basic Science in Medicine
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