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International Journal of Pediatrics، جلد ۲، شماره ۲.۳، صفحات ۵۷-۵۷
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عنوان انگلیسی The effect of Dimethyl Sulfoxide on hepatogenic differentiation of Mesenchymal Stem Cells
چکیده انگلیسی مقاله Background Adipose tissue mesenchymal stem cells (AT-MSCs) are suitable choices in treatment of liver associated diseases. Dimethyl Sulfoxide (DMSO) is an amphipathic molecule with potential of delivering both lipophilic and hydrophilic agents into cells. Few protocols used DMSO for induction of AT-MSCs towards hepatocyte like cells but the effect of DMSO on hepatogenic differentiation were not surveyed, previously. In the present study, we aimed at evaluation of the effect of DMSO in differentiation of AT-MSCs into hepatic lineage. Methods: We isolated MSCs from adipose tissue then multi-potency and surface markers of AT-MSCs was evaluated. Isolated AT-MSCs randomly dispensed in four groups including group 1: HGF treated, 2: HGF+DMSO treated, 3: HGF+ DMSO+ OMS treated, and group control for a period of 3 weeks in the expansion medium without serum, EGF and bFGF was also included in the first stage of inductions. The morphologic changes during induction period was observed with microscopy. The protein levels of albumin and a-fetoprotein (AFP) of the differentiating MSCs was investigated by ELISA and urea production was evaluated by colorimetric assay. The qRT-PCR was performed for quantitation of hepatocyte marker genes including a-fetoprotein (AFP), CK18, HNF4a, and HNF6. The glycogen storage of differentiated cells was visualized by periodic-acid Schiff‘s staining. Results: The results demonstrate that DMSO speeds up hepatic differentiation of AT-MSCs characterized by rapid changes in morphology, higher expression of hepatic marker gene (AFP) in both mRNA and protein level (P< 0.05), also, increased transcriptional levels of other hepatic genes including CK18, HNF4a, and HNF6 (P< 0.01) moreover, greater percentage of glycogen storage( p< 0.05) in DMSO treated groups. Conclusion: DMSO catalyzes hepatic differentiation, therefore using DMSO for acceleration of the hepatogenic protocols of AT-MSCs appears advantageous. Keywords: Adipose Tissue, Mesenchymal Stem Cells, Hepatic differentiation.
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نویسندگان مقاله عفت علیزاده | effat alizadeh
department of medical biotechnology, faculty of advanced medical science, tabriz university of medical science, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

نصرت اله ضرغامی | nosratollah zarghami
the umbilical cord stem cell research center ucsrc , tabriz university of medical science, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

محمدرضا باغبان اسلامی نژاد | mohammad reza baghaban eslaminejad
department of stem cells and developmental biology at cell science research center, royan institute for stem cell biology and technology, acecr, tehran, iran.

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

ابوالفضل اکبرزاده | abolfazl akbarzadeh
department of medical nanotechnology, faculty of advanced medical science, tabriz university of medical science, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

شهربانو جهانگیر | shahrbano jahangir
department of stem cells and developmental biology at cell science research center, royan institute for stem cell biology and technology, acecr, tehran, iran.

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

ابوالفضل برزگر | abolfazl barzegar
research institute for fundamental sciences rifs , university of tabriz, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

شهریار hahemzadeh | shahryar hahemzadeh
department of general surgery, faculty of medicine, tabriz university of medical science, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

ابوالقاسم محمدی | abolghasem mohammadi
department of agronomy and plant breeding, faculty of agriculture, university of tabriz, tabriz, iran.

سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

نشانی اینترنتی http://ijp.mums.ac.ir/article_2720.html
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