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Medical Journal of Islamic Republic of Iran، جلد ۱۳، شماره ۳، صفحات ۲۱۹-۲۲۳
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عنوان انگلیسی INTRACYTOPLASMIC SPERM INJECTION (lCSI) IN MICE BY A NEW HOLDING PIPETTE
چکیده انگلیسی مقاله Mice are ideal models for ICSI experiments because of the ease of culturing eggs/embryos in vitro and the availability of ample genetic information. Unfortunately, it has been extremely difficult. In this study we improved the mouse ICSI method by using a new holding pipette that was made of two pipettes such that one pipette was pulled and heat merged into the other one. The outer pipette had an outer diameter of 120 J..l. and an inner diameter of 80 J..l. to 85 J.1 (about mouse oocyte diameter). The inner pipette had an outer diameter of 80 µ to 85 µ. and an inner diameter of 55 µ to 60 µ. which was polished and narrowed to 20 J..l.on amicroforge. The distance between the tips of the two pipettes was adjusted to 120 µ. to 160 µ (1.5 to 2 fold longer than mouse oocyte diameter). Of 307 oocytes which were injected with a single spermatozoon, 206 (67.1 %) survived and 93 (45.1 %) of surviving oocytes showed normal fertilization (2 pronuclei and second polar body). Of 109 oocytes which were only sucked into the holding pipette (control group), 105 (96.3%) survived and only 4 (3.8%) of them became activated parthenogenically. U sing this new holding pipette, the oocyte is sucked into a glass tunnel and elongates to a reasonable length therefore the injection axis will be increased and piercing of the oolemma can be performed easily.
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نویسندگان مقاله امیر اسماعیل نژادمقدم | amir esmailnejad moghaddam
from the department of anatomy and embryology, medical school, mazandaran university of medical sciences, sari, i r. iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)

عباسعلی کریمپور ملکشاه | abbasali karimpour malekshah
from the department of anatomy and embryology, medical school, mazandaran university of medical sciences, sari, i r. iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)

masahiko hiroi | masahiko hiroi
the department of obstetrics and gynecology, medical school, yamagata university, yamagata, japan.


hidekazu سایتو | hidekazu saito
the department of obstetrics and gynecology, medical school, yamagata university, yamagata, japan.
نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-392&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Anatomical Sciences
نوع مقاله منتشر شده Original Research: Basic Science in Medicine
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