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Medical Journal of Islamic Republic of Iran، جلد ۱۳، شماره ۳، صفحات ۰-۰
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عنوان انگلیسی HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF THEOPHYLLINE IN HUMAN SERUM
چکیده انگلیسی مقاله A simple, rapid and sensitive HPLC method for the determination of theophylline (T) in human serum has been developed. An isocratic system consisting of a /-l Bondapak C18 column, mobile phase of methanol, phosphate buffer (22:78, pH=4.5), and a flow rate of 1.4 mL/min was used. The eluent was detected by UV at 275 nm at room temperature. 8-Chlorotheophylline (8-CT) was used as an internal standard. The serum samples deproteinated by methanol containing 8-CT and the supernatant was injected into the HPLC system. The retention times of 5.7 and 8.1 min were found for T and 8-CT, respectively. The linearity was checked in the range of 0.2- 30 µg/mL. Relative standard deviation for both inter-day and intra-day precision analysis was less than 5%. No interference was observed from endogenous serum components. Specificity was shown against some commonly co-administered drugs. Simple and fast sample preparation, small sample volume (250 µL), precision, reproducibility, specificity, sensitivity and high percentage recovery (98 %) make the method to be practically useful for T monitoring in asthmatic patients.
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نویسندگان مقاله h tajerzadeh | h tajerzadeh
from the biopharmacy division, faculty of pharmacy, tehran university of medical sciences, tehran, ir.iran, 14155 6451.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

s صدرای | s sadray


نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-388&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Pharmacology
نوع مقاله منتشر شده Original Research
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