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Iranian Journal of Biotechnology، جلد ۱۴، شماره ۱، صفحات ۵۱-۵۵
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عنوان انگلیسی Effect of Antioxidants, Amino Acids and Plant Growth Regulators on in vitro Propagation of Rosa centifolia
چکیده انگلیسی مقاله Background: Rosa centifoliais commercially propagated by asexual means but in vitro propagation ensure the production of disease free and healthy plants and browning of explants creates hurdle in their multiplication. Objectives: The aim was to reduce oxidative browning of shoots of R. centifolia in MS medium during in vitro propagation. Materials and Methods: Axillary buds of R. centifolia were sterilized with 70% ethyl alcohol for 4 min and 5% sodium hypochlorite for 2 min followed by three washing with sterilized double distilled water. In order to control oxidative browning, Ascorbic acid (100 mg.L-1), citric acid (100 mg.L-1) and activated charcoal (3 g.L-1) were used while to control withering of shoots, different concentrations (3.0 mg.L-1, 6.0 mg.L-1, 9.0 mg.L-1) of either glutamine, asparagine and proline were put into trial. Different concentrations of Benzyl aminopurine (BAP) and naphthalene acetic acid (NAA) were used for in vitro shoot and root formation. Results: Minimum browning percentage (20%) was achieved in the presence of activated charcoal (3.0 g.L-1) and pretreatment of explants with running tap water. Asparagin (9.0 mg.L-1) produced maximum shooting (93%), minimum withering (6.67%), and it took longer period (27 days) for shoots to wither. BAP (3.0 mg.L-1) + NAA (0.5 mg.L-1) was produced the highest number of shoots (1.63), in a shortest periods (9 days). For root production, NAA (1.5 mg.L-1) + BAP (0.5 mg.L-1) reduced the time to 11 days with maximum number of roots (4.33) and root length (4.20 cm). Conclusions: The supplement of activated charcoal (3.0 g.L-1), a sparagin (9.0 mg.L-1) and combination of BAP and NAA in the MS medium is effective for in vitro propagation of R. centifolia.
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نویسندگان مقاله gulzar اختر |
department of horticulture, university college of agriculture, university of sargodha, sargodha, pakistan

محمد جعفر jaskani | muhammad jafar
institute of horticultural sciences, university of agriculture, faisalabad, pakistan

یاشار سجاد |
department of environmental sciences, comsats institute of information technology, abbottabad, pakistan

احسن اکرم |
department of horticulture, university college of agriculture, university of sargodha, sargodha, pakistan

نشانی اینترنتی http://www.ijbiotech.com/article_13565_e7ebe2f731c3079579edc8f9b9855d08.pdf
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