این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Medical Journal of Islamic Republic of Iran، جلد ۱۲، شماره ۱، صفحات ۴۷-۵۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی ISOLATION AND PURIFICATION OF MAJOR OUTER MEMBRANE PROTEINS FROM BRUCELLA ABORTUS S-99
چکیده انگلیسی مقاله Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminants. The OMP preparation was purified by column chromatography on Sephacryl S-200. Three major symmetrical peaks emerged from the column with kav values of 1. 81, 2.42 and 2.56 in succession in addition to a few closely related minor peaks. Characterization of crude OMP on SDS-PAGE showed 13 protein bands. The three major peaks 1,2 and 3 were subjected to SDS-PAGE separately and the molecular weights of peaks 2 and 3 were calculated to be 26 and 38 kDa, respectively and the first peak was further resolved into two subfractions with molecular weights of 62 and 67 kDa. However, after treatment of OMP with trypsin the number of bands were reduced to one prominent band with a molecular weight of 38 kDa and a thinner band of 41 kDa.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله فرشته شاهچراغی | fereshteh shahcheraghi
from the department of microbiology, medical school, tarbiat modaress university, tehran,

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

محمد باقر اسلامی | mohammad bagher eslami
from the department of microbiology, medical school, tarbiat modaress university, tehran,

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

بهمن تبرایی | bahman tabaraie
the department of bacterial vaccine and antigen production, pasteur institute of iran, tehran, islamic republic of iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

قربان بهزادیان نژاد | ghorban behzadian nejad
from the department of microbiology, medical school, tarbiat modaress university, tehran,

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-496&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Microbiology and Anatomy
نوع مقاله منتشر شده Original Research: Basic Science in Medicine
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات