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Medical Journal of Islamic Republic of Iran، جلد ۱۱، شماره ۳، صفحات ۲۲۹-۲۳۶
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چکیده فارسی مقاله
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عنوان انگلیسی EXTRACELLULAR LIPA SE ACTIVITY CHARACTERIZATION OF SOME PSEUDOMONAS AERUGINOSA STRA INS ISOLATED FROM HUMAN INFECTIONS
چکیده انگلیسی مقاله Pseudomonas aeruginosa EF2, ATCC 9027 and ATCC 19660 were grown in a continuous culture under Tween 80 (polyoxyethylene sorbitan monooleate) limitation and optimum conditions (pH 6.5, 37°C at dilution rate of 0.05/h). Culture supernatants were carefully removed and stored at -20°C. To purify the lipases, the culture supernatant was reduced in volume to approximately 10 mL by an ultrafiltration unit. Excess salts were removed and extracellular lipase was purified. Biochemical characterization and SDS polyacrylamide gel electrophoresis suggested that lipase particles consisted of protein and carbohydrate-including lipopolysaccharide-with the major enzyme activity being lipase. Lipase activity was measured as the rate of standard olive oil (predominantly triolein) hydrolysis. Characterization of the purified extracellular lipases was then investigated by hydrolysis activity, interesterification reactions and effect on the chemotaxis and chemiluminescence reactions on human peripheral blood neutrophils and monocytes. It was shown that lipase from the EF2 strain was the most effective enzyme used and monocytes were much more sensitive to lipases than neutrophils. Since monocytes are one of the most important cells of the host defence system, lipase activity of Pseudomonas aeruginosa may contribute to the pathogenesis of infections caused by this Gram-negative bacterium.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله محمد حجارتبار | mohammad hajjartabar
from the department of microbiology, school of medical sciences, tarbiat modarres university

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

ناصر قایمی | nasser ghaemi
, the faculty of sciences, tehran university, tehran, islamic republic of iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نسرین معظمی | nasrin moazzami
from the department of microbiology, school of medical sciences, tarbiat modarres university

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

مهشید faghlhi شیرازی | mahshid faghlhi shirazi
the food research institute, vic 3030, australia.
نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-356&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Microbiology and Anatomy
نوع مقاله منتشر شده Original Research: Basic Science in Medicine
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