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Medical Journal of Islamic Republic of Iran، جلد ۱۱، شماره ۲، صفحات ۱۳۷-۱۴۱
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عنوان انگلیسی RAPID IDENTIFICATION OF Vibrio cholera-01 BY COAGGLUTINATION TEST USING MONO-SPECIFIC ANTIBODY
چکیده انگلیسی مقاله Mono-specific antisera against Vibrio cholera Ogawa NIH-43 and Vibrio cholera Inaba 35-A3 were prepared from rabbit hyperimmune sera by absorbing against a heterologous strain. Using ammonium sulphate precipitation procedure, gamma globulins were purified and concentrated. To visualize antigen-antibody reaction, gamma globulins were conjugated to Staphylococcus aureus cowan-l (NCTC: 8325) in the presence of 50% propanol- 1. Then equal suspensions of each conjugated serum were mixed to prepare V. cholera. Rectal swab samples from suspected choleric patients were inoculated in bile peptone broth for 5 hours at 37°C. One drop of each sample was mixed with one drop of VBCR and coagglutination was read at 2-3 minutes. The results were compared with corresponding results obtained from conventional culture methods. Specificity and sensitivity of coagglutination tests were found to be 98.03% and 95.1 %, respectively. Regarding the fact that rapid diagnosis of cholera is vital to save patients, our study reveals that coagglutination test, using bivalent mono-specific antisera, can be considered as a simple, rapid and reliable test to detect V. cholera-O 1 from stool samples of suspected patients.
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نویسندگان مقاله سید عباس بازرگان | seyed abbas bazargan
from the department of microbiology, medical school, tarbiat modarres university, tehran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

بهمن تبرایی | bahman tabaraie
the department of bacterial vaccine and antigen production, p asteur institute of iran


بهرام فتح اله زاده | bahram fatollahzadeh
from the department of microbiology, medical school, tarbiat modarres university, tehran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

نسرین معظمی | nasrin moazzami
the biotechnology department, iranian research organization
نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-378&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Microbiology and Anatomy
نوع مقاله منتشر شده Original Research: Basic Science in Medicine
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